We narrowed to 5,326 results for: pcdna3.1

1. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

   Plasmid

   #112157

   Purpose

   Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

   Depositor

   Ben Giepmans

   Article

   de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

   Insert

   anti-GFP FLIPPER-body
   

   Tags

   His and Thrombin cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 CMV mCherry-eGFP BGH

   Plasmid

   #92280

   Purpose

   Control FRET construct; tandem mCherry-eGFP fusion

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   mCherry-eGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-KozATG-dL5-2XG4S-actin

   Plasmid

   #73262

   Purpose

   Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

   Insert

   KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
   

   Tags

   The FAP and actin are fused with 2 copies of a G4…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

   Plasmid

   #225587

   Purpose

   Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-vhhGFP4-T2A-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/nV5-DEST-beta catenin

   Plasmid

   #20140

   Depositor

   Valeri Vasioukhin

   Article

   Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

   Insert

   pcDNA3.1/nV5-DEST-beta catenin (Ctnnb1 Mouse)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   T3I

   Promoter

   CMV

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

   Plasmid

   #158952

   Purpose

   Express human RAD51(F129A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed phenylalanine 129 to alanine

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

   Plasmid

   #171432

   Purpose

   Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-hH3
   

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

   Plasmid

   #114111

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

   Insert

   Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

   Plasmid

   #171427

   Purpose

   Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-FRB
   

   Expression

   Mammalian

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/FGFR2-S252W(Apert) myc

    Plasmid

    #33249

    Depositor

    Alka Mansukhani, Claudio Basilico

    Article

    Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

    Insert

    FGFR2 (Fgfr2 Mouse)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Mutation S252W in FGFR2 found in Apert syndrome. …

    Promoter

    CMV

    Available Since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1/CIP2A(1-905) L533E V5 His

    Plasmid

    #119288

    Purpose

    CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    L533E

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

    Plasmid

    #193997

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181_NLS

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181_NLS
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

    Plasmid

    #127314

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

    Plasmid

    #163979

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

    Plasmid

    #62975

    Purpose

    Expresses HA-tagged rat SOX9 residues 286-507

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Tags

    HA (YPYDVPDY)

    Expression

    Mammalian

    Mutation

    codes for residues 286-507

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid

    #216309

    Purpose

    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

    Plasmid

    #166791

    Purpose

    Mammalian cell expression vector for His-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Tags

    HIS-TEV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Available Since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

    Plasmid

    #89636

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    S317A, AGC-->GCC

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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