We narrowed to 7,531 results for: ski

1. pCAGGS D614G soluble Spike

   Plasmid

   #164651

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pscALPSpuro-SsACE2 (Pig)

   Plasmid

   #158085

   Purpose

   Expresses pig ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Pig ACE2 (ACE2 Sus scrofa)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pscALPSpuro-ClACE2 (Dog)

   Plasmid

   #158083

   Purpose

   Expresses dog ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Dog ACE2 (ACE2 )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPSpuro-RnACE2 (Rat)

   Plasmid

   #158086

   Purpose

   Expresses rat ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Rat ACE2 (Ace2 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscALPSpuro-FcACE2 (Cat)

   Plasmid

   #158082

   Purpose

   Expresses cat ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Cat ACE2 (ACE2 )
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCIDT-attL1-Human ABeta-attR5

   Plasmid

   #160436

   Purpose

   Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Homo sapiens amyloid beta precursor protein (APP Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQC MCS IRES Puro

   Plasmid

   #110343

   Purpose

   γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113702

   Purpose

   Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-syn-FLEX-axon-jYCaMP1s

   Plasmid

   #135421

   Purpose

   Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Tags

   GAP43 axon targeting sequence

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX-FKBP-Kras-Blasticidin

    Plasmid

    #120714

    Purpose

    Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158-188

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LENTICRISPR-GPI_sgRNA1

    Plasmid

    #201594

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GPI (GPI Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113687

    Purpose

    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113688

    Purpose

    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV Cdk2ap1CAN-HA

    Plasmid

    #178030

    Purpose

    Retroviral vector for the purpose of overexpression in mammalian cell culture

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    GAG

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXS-IRES-Blast POLE1 C-ISCmut

    Plasmid

    #160804

    Purpose

    Express C-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 C-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113689

    Purpose

    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

    Plasmid

    #51249

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 980,1006 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pQC MCS IRES G418

    Plasmid

    #110344

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

    Plasmid

    #51247

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only