162,552 results

1. Flag-FOXP1

   Plasmid

   #153145

   Purpose

   Expression of Flag tagged human FOXP1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXP1 (FOXP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-entry-puro

   Plasmid

   #85745

   Purpose

   Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

   Plasmid

   #187583

   Purpose

   Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PML (PML Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-FLAG-GFP-OSBP

   Plasmid

   #134659

   Purpose

   Flag and GFP-tagged OSBP

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   OSBP (OSBP Human)
   

   Use

   Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-KIR3DL2-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156758

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR3DL2 (KIR3DL2 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB402SC

   Plasmid

   #224552

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Use

   Tags

   Twin-Strep-HA

   Expression

   Plant

   Mutation

   Promoter

   2 X 35S

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB402SN

   Plasmid

   #224551

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Use

   Tags

   HA-Twin-Strep

   Expression

   Plant

   Mutation

   Promoter

   2 X 35S

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET tdTomato LIC cloning vector (H6-tdTomato)

   Plasmid

   #86928

   Purpose

   pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and td…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

   Plasmid

   #196227

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn iGluSnFR3 v857.NGR
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and NGR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0057

    Plasmid

    #142530

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MAF (MAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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