165,688 results

1. pRK5-HA-Ubiquitin-K6

   Plasmid

   #121151

   Purpose

   Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Expression

   Mammalian

   Mutation

   All Ks apart from K6 mutated to Rs

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

   Plasmid

   #193851

   Purpose

   E1b promoter for transgenic enhancer assays in zebrafish

   Depositor

   Junsu Kang

   Article

   Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Zebrafish expression

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

   Plasmid

   #180012

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBES1-LacOx50-mCh-bla

   Plasmid

   #210260

   Purpose

   Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   mCherry
   

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCre-P2A-mCherry

   Plasmid

   #212105

   Purpose

   Expression of Cre and mCherry in mammalian cells

   Depositor

   Daekee Lee

   Insert

   Cre recombinase and mCherry
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPRi

   Plasmid

   #199808

   Purpose

   vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

   Depositor

   Steve Salipante

   Article

   Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

   Inserts

   dCas9
   sgRNA with GFP cassette
   

   Use

   CRISPR

   Promoter

   P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pArcCreER

   Plasmid

   #46389

   Depositor

   Liqun Luo

   Article

   Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

   Inserts

   Arc (Arc Mouse)
   CreERT2
   

   Use

   Mouse Targeting

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPdX

   Plasmid

   #85084

   Purpose

   Expresses putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 73 to Serine and Cysteine 85 to …

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. actc1b-2PH-PLCd-mKate2

    Plasmid

    #109500

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-2PH-PLCd-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge