168,839 results
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Plasmid#233614PurposeExpression of Rluc-Fluc reporter gene containing no YDepositorInsertRluc-Y0x-Fluc
UseLuciferaseAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
SeqCBE
Plasmid#232115PurposeExpresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP
Plasmid#232608PurposePan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTRDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-5xQUAS+Δpes-10P::AI::eTsChR
Plasmid#232612PurposeExpression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric proteinDepositorInserteTsChR
ExpressionWormMutationN/AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA Q67H/N74D
Plasmid#217442PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).DepositorInsertgag/pol
UseLentiviralMutationCA Q67H,N74DAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B G120A-TdLanYFP
Plasmid#228561PurposeEncodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CRISPRi-sgRNA
Plasmid#221135PurposeHelper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with dCas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CRISPRi
Plasmid#221136PurposeCoxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.DepositorInsert3xF-dCas9
UseCRISPRTags3xFLAGExpressionBacterialPromotercbu1169 promoterAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-V5-n2StayGoldC4 (JDW 1354)
Plasmid#224486PurposeGateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)DepositorInsertH2B-V5-n2StayGoldC4
UseGateway cloningAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only