We narrowed to 7,112 results for: ef1a

1. TFORF2600

   Plasmid

   #144213

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU6F2 (POU6F2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF2783

   Plasmid

   #143616

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GLIS3 (GLIS3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF2381

   Plasmid

   #143367

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MBD1 (MBD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2507

   Plasmid

   #144123

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PML (PML Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF1339

   Plasmid

   #144172

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU3F4 (POU3F4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX307-HCoV-NL63-3CL-C144A

   Plasmid

   #160287

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF0141

   Plasmid

   #142462

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU5F1B (POU5F1B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF2551

   Plasmid

   #142323

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BID (BID Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MLM3709

   Plasmid

   #49943

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CD RH-3 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

10. TFORF2805

    Plasmid

    #144241

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TAF2 (TAF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLEX307-SARS-CoV-3CL-C145A

    Plasmid

    #160281

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO_AU1.HA.VSVg_mCherry-NLS

    Plasmid

    #178220

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO_AU1.HA.S_mCherry-NLS

    Plasmid

    #178219

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.S and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO_AU1.HA.HSV_mCherry-NLS

    Plasmid

    #178216

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.HSV and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO_AU1.C.VSVg_mCherry-NLS

    Plasmid

    #178214

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_AU1.C.S_mCherry-NLS

    Plasmid

    #178213

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.S and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_AU1.C.HSV_mCherry-NLS

    Plasmid

    #178210

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.HSV and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF3172

    Plasmid

    #144648

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_S.FLAG.VSVg_NGFR

    Plasmid

    #158345

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.FLAG.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0696

    Plasmid

    #141665

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF3A (GTF3A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBbi-Pur-mCerulean3-cpVenus[E172]

    Plasmid

    #125203

    Purpose

    SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high control

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    mCerulean3-cpVenus[E172]
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

23. TFORF3122

    Plasmid

    #144598

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SATB1 (SATB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF3092

    Plasmid

    #144568

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NPAS1 (NPAS1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF2023

    Plasmid

    #144323

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NPAS3 (NPAS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF2024

    Plasmid

    #144324

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NPAS3 (NPAS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF1715

    Plasmid

    #142833

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LDB2 (LDB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF1713

    Plasmid

    #142832

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LDB2 (LDB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF0819

    Plasmid

    #141719

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    LHX5 (LHX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF3016

    Plasmid

    #144492

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MLX (MLX Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF1170

    Plasmid

    #141801

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MLX (MLX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCEFL GAL4dbd-KLF4 1-329

    Plasmid

    #140146

    Purpose

    GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    KLF4 Kruppel factor (KLF4 Human)
    

    Use

    Tags

    GAL4dbd

    Expression

    Mammalian

    Mutation

    Deleted amino acids 330 to 479

    Promoter

    EF1a

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLKO_NWS.Ollas.S_mCherry-NLS

    Plasmid

    #178279

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.Ollas.S and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO_HSV.S.V5_mCherry-NLS

    Plasmid

    #178278

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.S.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO_HSV.Ollas.V5_mCherry-NLS

    Plasmid

    #178277

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.Ollas.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO_HSV.NWS.S_mCherry-NLS

    Plasmid

    #178273

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.S and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MLM3747

    Plasmid

    #49965

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-6 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

38. TFORF3385

    Plasmid

    #144861

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO_HSV.FLAG.HA_NGFR

    Plasmid

    #158294

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HSV.FLAG.HA

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJFA345D4

    Plasmid

    #49238

    Purpose

    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
    

    Use

    TALEN; Tale-tet1 expression vector

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. MLM3743

    Plasmid

    #49962

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-5 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

42. TFORF0132

    Plasmid

    #142461

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FIZ1 (FIZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TFORF2021

    Plasmid

    #142314

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NPAS4 (NPAS4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TFORF3266

    Plasmid

    #144742

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF213 (ZNF213 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TFORF3098

    Plasmid

    #144574

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF467 (ZNF467 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. TFORF2285

    Plasmid

    #142009

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HES3 (HES3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. TFORF2097

    Plasmid

    #141920

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TFORF2075

    Plasmid

    #141908

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYB (MYB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLV V5-TbID-emerin

    Plasmid

    #175099

    Purpose

    Lentiviral expression of V5-TbID-tagged mouse Emd

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5-TbID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. TFORF2320

    Plasmid

    #142033

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TAF1A (TAF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge