We narrowed to 7,223 results for: ALP

1. pmcherry α-catenin ΔVBS

   Plasmid

   #178649

   Purpose

   express α-catenin without a vinculin binding site (VBS)

   Depositor

   Daniel Conway

   Article

   Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

   Insert

   α-catenin (CTNNA1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   VBS of alpha catenin was removed and replaced wit…

   Promoter

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pATZ-BFP-HA-GFP11

   Plasmid

   #177721

   Purpose

   ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11, HA, and TagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   E342K (ATZ mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-IpgD(C439S)

   Plasmid

   #183667

   Purpose

   N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C439S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. iSH-iRFP670-sspB

   Plasmid

   #176102

   Purpose

   Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   phosphatidylinositol 3-kinase (Pik3r1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro

   Plasmid

   #22135

   Depositor

   William Kaelin

   Article

   Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids from wt: Proline 405 t…

   Promoter

   Available since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. α-catenin ECFP-YPET FRET ΔF-actin binding site

   Plasmid

   #198245

   Purpose

   Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Deleted F-actin binding site on a-catenin (849-90…

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ Zf ctnna1L344P

   Plasmid

   #185407

   Purpose

   Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   ctnna1 (ctnna1 Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 344 to Proline

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag

   Plasmid

   #185682

   Purpose

   Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 (ERN1 Synthetic, Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   K599A

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEBG-bZIPa

    Plasmid

    #12436

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    bZIP of C/EBPa (CEBPA Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

11. XE237 Human LEF-1 pCS2+

    Plasmid

    #16709

    Depositor

    Randall Moon

    Article

    Li et al J Biol Chem. 2004 Jan 23;279(4):2873-84.

    Insert

    Human LEF-1 (LEF1 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

    Plasmid

    #185681

    Purpose

    Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of lumenal domain

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag

    Plasmid

    #185684

    Purpose

    Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    K121Y

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SopB-EGFP

    Plasmid

    #183655

    Purpose

    C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pESC-LEU-SopB

    Plasmid

    #183670

    Purpose

    Inducible Salmonella Typhimurium SopB for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pESC-Leu-SopB(C460S)

    Plasmid

    #183671

    Purpose

    Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C460S

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW TetO HA-AMPKa1 AS

    Plasmid

    #69825

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

    Plasmid

    #185685

    Purpose

    Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of linker, kinase, and Rnase domains

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-7 asyn S87E

    Plasmid

    #36059

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-7 asyn S87A

    Plasmid

    #36058

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87A (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87A

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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