We narrowed to 4,126 results for: Alk

1. nSaCas9 I744 miniABEMax V82G

   Plasmid

   #135379

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 I744 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-Nedd1-mNeonGreen

   Plasmid

   #196864

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mNeonGreen (Nedd1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MKV937/nlp-1p::FlincG3

   Plasmid

   #140508

   Purpose

   This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

   Depositor

   Miri VanHoven

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   nlp-1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsNot8-D40AE42A_AH

   Plasmid

   #148902

   Purpose

   Mammalian Expression of HsNot8-D40AE42A

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

   Insert

   HsNot8-D40AE42A (CNOT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX131

   Plasmid

   #167149

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   4'-phosphopantetheinyl transferase, npgA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. S1 1029 CP ABEMax strategy 1

   Plasmid

   #135357

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX028

   Plasmid

   #167151

   Purpose

   MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnHispS-ocsT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   p35s

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX018

   Plasmid

   #167144

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal luciferase, nnLuz
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

   Plasmid

   #62577

   Purpose

   Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pX029

    Plasmid

    #167152

    Purpose

    MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnH3H-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX019

    Plasmid

    #167145

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal hispidin-3-hydroxylase, nnH3H
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AIDx-ID-1058

    Plasmid

    #135347

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-1058-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIR-Report-RASA1-3'UTR

    Plasmid

    #62575

    Purpose

    Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Luciferase

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

    Plasmid

    #231350

    Purpose

    Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

    Plasmid

    #231352

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

    Plasmid

    #231353

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

    Plasmid

    #231356

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

    Plasmid

    #231357

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge