We narrowed to 165,550 results for: addgene

1. gRNA4 _8xCTS-ECFP-pA

   Plasmid

   #200246

   Purpose

   Mammalian transfections; 8xCTS-ECFP reporter construct 4

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   sgRNA4_8xCTS
   

   Expression

   Mammalian

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK BirA* zeo

   Plasmid

   #192332

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-BirA

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BirA*
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN_TT BirA* w ccdB

   Plasmid

   #192310

   Purpose

   Gateway entry vector for cloning

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   BirA*
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT 3xFLAG-Linker

   Plasmid

   #192305

   Purpose

   Gateway entry vector for cloning

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   Linker
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Venus-Bik-L61G-pEGFP-C1

   Plasmid

   #177426

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Leucine 61 replaced with glycine

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Venus-Bik-GG153AA-pEGFP-C1

   Plasmid

   #177427

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Glycines 153 and 154 mutated to alanine

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Venus-Bik-G154STOP-pEGFP-C1

   Plasmid

   #177428

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Glycine 154 replaced with STOP codon

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. NODAL-T7TS

   Plasmid

   #115250

   Purpose

   For in vitro transcription of human NODAL open reading frame RNA

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. NODALvar-T7TS

   Plasmid

   #115251

   Purpose

   For in vitro transcription of a human NODAL splice variant open reading frame RNA

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL splice variant (NODAL Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. H2B-MiniE-mEm

    Plasmid

    #181991

    Purpose

    MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    H2B-MiniE-mEm
    

    Tags

    mEmerald

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6DXH (USP05)

    Plasmid

    #119968

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    USP05 (USP5 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 171-290

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 6DUB (LOC149281)

    Plasmid

    #119967

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    LOC149281 (METTL11B Human)
    

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 58-278

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. FOXN1 (FOXN1A-c017)

    Plasmid

    #110249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FOXN1 (FOXN1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 5T1G (CBX1)

    Plasmid

    #101884

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX1 (CBX1 Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. 2QO2 (EPHA3)

    Plasmid

    #101271

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPHA3 (EPHA3 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDHJS4 AN-

    Plasmid

    #78247

    Purpose

    One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

    Inserts

    attB
    attP
    

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pDHJS4 AN+

    Plasmid

    #78246

    Purpose

    One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

    Inserts

    attB
    attP
    

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCVD630

    Plasmid

    #66163

    Purpose

    cloning of ace gene

    Depositor

    James Kaper

    Article

    Trucksis et al Proc Natl Acad Sci U S A. 1993 Jun 1;90(

    Insert

    ace
    

    Use

    Cloning vector

    Promoter

    Unknown

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. MXS_PA-GFP

    Plasmid

    #62412

    Purpose

    MXS_chaining vector with PA-GFP

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    PA-GFP
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_loxP

    Plasmid

    #62430

    Purpose

    MXS_chaining vector with loxP

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    loxP site
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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