Addgene

We narrowed to 2,414 results for: organization Addgene

Showing: 1861 - 1880 of 2414 results

Results per page:

Sort results by:

pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

Plasmid

#127356

Purpose

gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, V5, and mVenus

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-mTurq2-Apollo-NADP+

Plasmid

#193303

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Use

Tags

mTurqoise2 and mitochondrial targeting sequence

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cyto-mTurq2-Apollo-NADP+

Plasmid

#193301

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Use

Tags

mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-TMEM192-FLAG

Plasmid

#164977

Purpose

Lentiviral vector for TMEM192 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry C1 MFF

Plasmid

#157760

Purpose

Expression of mcherry:MFF in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

MFF (MFF Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

TRE3G

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag2-Sox9 T236A

Plasmid

#111455

Purpose

Transient expression of Flag-tagged human Sox9 with T236A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T236A mutation

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BE3-FNLS(RA)

Plasmid

#112671

Purpose

CMV expression vector for BE3-FNLS construct (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus and D10A

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG

Plasmid

#176396

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

GAL1 promoter, SZ1-RGG-GFP-RGG-RGG

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Ef1a-SunTag-RPS7-PP7-WPRE

Plasmid

#198338

Purpose

PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

GCN4x24-RPS7-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops and SunTag

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Nuc-mTurq2-Apollo-NADP+

Plasmid

#193302

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Use

Tags

SV40 NLS and mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-NPC1(D786N)-FLAG

Plasmid

#164975

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D786N, insertion L aa15 (please see depositor co…

Promoter

EF1a

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-TRE3G-BE3-PGK-Puro

Plasmid

#110845

Purpose

Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

TRE3G

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Tag2-Sox9 T240A

Plasmid

#111457

Purpose

Transient expression of Flag-tagged human Sox9 with T240A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T240A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge