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Plasmid#87329PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFlagCMV-mCALEBshort
Plasmid#89398Purposemammalian expression of mouse CSPG5 short isoformDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCSPG5 (Cspg5 Mouse)
TagsFlagExpressionMammalianMutationshort isoformPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-XhoI
Plasmid#87330PurposeTo express shRNA against human Nup93DepositorInsertshRNA against human Nup93
UseRNAiExpressionMammalianPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a W118S
Plasmid#40042DepositorInserthSlp4-a W118S (SYTL4 Human)
TagsEGFPExpressionMammalianMutationTryptophane 118 to SerinePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
Srs2-C-terminus_pET28a
Plasmid#247774PurposeThe primary use of this plasmid is as a target for CRISPR-Cas9. It can also be used to express the His-tagged C-terminus of Srs2 with Lys-to-Arg mutations.DepositorInsertSrs2-C-terminus (NEWENTRY Budding Yeast)
MutationThe C-terminus of the gene has Lys-to-Arg mutatio…PromoterT7Available sinceMay 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
KG#797
Plasmid#110916PurposeExpresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neuronsDepositorInsertsunc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
TagsGFP (contains 3 artificial introns)ExpressionBacterialAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK600
Plasmid#71605PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, histidine…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-CI2F
Plasmid#191254PurposeExpresses chymotrypsin inhibitor with an N-terminal MBP fusion proteinDepositorInsertChymotrypsin Inhibitor 2
TagsMaltose Binding ProteinExpressionBacterialAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin W403A
Plasmid#227357PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin W403A (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pR-mTagBFP2
Plasmid#228596PurposeMammalian expression of mTagBFP2, for MuSIC barcode constructionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTagBFP2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mRuby3
Plasmid#228609PurposeMammalian expression of mRuby3, for MuSIC barcode constructionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRuby3
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Neuroligin-3 GFP/pCMV
Plasmid#197338PurposePlasmid expressing an antigen targeting Neuroligin-3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNeuroligin3 (Nlgn3 Mouse)
TagsmVenusExpressionMammalianAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE1
Plasmid#195367PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAGDepositorInserthA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten plus 3…Tags3xflagExpressionPlantPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS
Plasmid#194750PurposeTo fuse your protein of interest to the N-terminus of GPI-anchored-BioID2DepositorTypeEmpty backboneTagsHAExpressionMammalianAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKTop::sptse5-CT (1169-1229)
Plasmid#192948PurposeFor membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1229)
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1229)Available sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProGL2 GW
Plasmid#80689Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneExpressionPlantPromoterProGL2Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA1-EF1α-sfGFP150TAG-4xtRNA
Plasmid#251517PurposeExpression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cellsDepositorInsertsfGFP150TAG
TagsHis6 and V5ExpressionMammalianMutation150 TAG (amino acid 150 mutated to stop codon)PromoterEF1alphaAvailable sinceMarch 12, 2026AvailabilityAcademic Institutions and Nonprofits only