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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205550

Purpose

Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Bxb1-LP-v2-TC

Plasmid

#194326

Purpose

AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE47

Plasmid

#90385

Purpose

IFNB1 - Interferon beta gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IFNB1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8m

Plasmid

#169248

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hSyn1-GFP

Plasmid

#177810

Purpose

Encodes a GFP driven by human synapsin I promoter

Depositor

Christopher Grigsby

Article

Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

Insert

CopGFP inserted behind human synapsin I promoter (SYN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Syn1

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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mito-meGFP

Plasmid

#172481

Purpose

meGFP targeted to the mitochondrial matrix with a human COX8 presequence

Depositor

Thomas Schwarz

Insert

Mito

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Go1-CASE

Plasmid

#168123

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at L91/G92 within GNAO1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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