We narrowed to 5,173 results for: pcDNA3.1+

1. pcDNA3-DN-hCUL5-FLAG

   Plasmid

   #15823

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin5 (CUL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N441

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-TRBP

   Plasmid

   #15666

   Depositor

   Dong-Yan Jin

   Article

   Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

4. VN-gamma Synuclein

   Plasmid

   #89733

   Purpose

   expresses human gamma Synuclein fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

   Insert

   SNCG (SNCG Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-DN-hCUL2-FLAG

   Plasmid

   #15819

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005 . 399():287-309.

   Insert

   Cullin2 (CUL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N427

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mVenus-hCOL1A1

   Plasmid

   #140111

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRPML2-YFP

   Plasmid

   #18830

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML2 (Mcoln2 Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSB-CMV-MCS-Puro dr_cPLA2-mKate2

   Plasmid

   #163844

   Purpose

   Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Bongwoori-Pos6

   Plasmid

   #101655

   Purpose

   Bongwoori with the CC1-Pos6 linker length and composition.(* super ecliptic pHluorin A227D)

   Depositor

   Bradley Baker

   Article

   Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

   Insert

   Bongwoori-Pos6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRPML3-HA

    Plasmid

    #18832

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML3 (Mcoln3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 Plk4(Sak) wt (Nigg HR9)

    Plasmid

    #41165

    Depositor

    Erich Nigg

    Article

    Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-PalmPalm-ClopHensor

    Plasmid

    #25940

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    PalmPalm-ClopHensor
    

    Use

    Tags

    Gap20 and Strep-II

    Expression

    Mammalian

    Mutation

    N-term (20AA) of Gap-43

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZipGFP11_TEV

    Plasmid

    #81243

    Purpose

    Zipped GFP11 with TEV cleavage sequence

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    zipped GFP11 with TEV cleavage sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FlagMBNL3

    Plasmid

    #96901

    Purpose

    expression of Flag-tagged human MBNL3

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human MBNL3 cDNA (MBNL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mApple-hCOL1A1

    Plasmid

    #140113

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-mouse PKA-RIalpha-mEGFP

    Plasmid

    #45525

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RI alpha tagged by mEGFP
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3-mouse PKA-RIIalpha-mEGFP

    Plasmid

    #45527

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII alpha tagged by mEGFP
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-Nup37-EGFP-polyA

    Plasmid

    #104562

    Purpose

    Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Nup37 (Nup37 Mouse)
    

    Use

    Tags

    EGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEQ1180

    Plasmid

    #83805

    Purpose

    express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

    Depositor

    Adam Geballe

    Article

    Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

    Insert

    TRS1
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

21. JunD

    Plasmid

    #187904

    Purpose

    Expresses JunD in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    JunD (Jund Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. VinculinTS-A50I

    Plasmid

    #111827

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Plk1 FRET sensor c-jun substrate

    Plasmid

    #45203

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

    Insert

    Plk1 FRET sensor with c-jun substrate
    

    Use

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZZMP122

    Plasmid

    #231756

    Purpose

    Mammalian expression of SPO11-YF

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    SPO11-YF (Spo11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y138F

    Promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZZMP154

    Plasmid

    #231760

    Purpose

    Mammalian expression of LZ-SPO11

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    LZ-SPO11 (Spo11 Mouse, Budding Yeast)
    

    Use

    Tags

    Flag-LeucineZipper

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-hBcan

    Plasmid

    #18966

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Jul 7. ():.

    Insert

    Brevican (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

27. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Opto-hEGFR_308

    Plasmid

    #58746

    Purpose

    Expresses Opto-hEGFR (light-activated receptor tyrosine kinase)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    Opto-hEGFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mV-NcVV-LOV_231

    Plasmid

    #58689

    Purpose

    Expresses mVenus-fusion protein of NcVV-LOV domain (Neurospora crassa Vivid)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    mV-NcVV-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmyc-GFP-TNRC6C

    Plasmid

    #42023

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6C (TNRC6C Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge