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Showing: 4701 - 4750 of 16884 results

  14. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  28. pDONR223_JAK2_WT

    Plasmid
    #81756
    Purpose
    Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.
    Depositor
    William Hahn, David Root, Jesse Boehm
    Article
    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi
    Insert
    JAK2 (JAK2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    V617F
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    81756-plasmid-map-sequence-id-168863

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  37. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

    Plasmid
    #169161
    Purpose
    To express nsp10-nsp14 fusion protein in E. coli
    Depositor
    John Diffley
    Article
    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464
    Insert
    14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    Use
    Tags
    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…
    Expression
    Bacterial
    Mutation
    Codon optimised for E. coli
    Promoter
    T5
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169161-plasmid-map-sequence-id-333990

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Showing: 4701 - 4750 of 16884 results