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pCDB285

Plasmid

#80687

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEEH_04

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pPICZ:hTRAAK(G124I)

Plasmid

#130672

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, G124I

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Rhoc-m_3'UTR

Plasmid

#31885

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc mutant 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-302b, 372 7-mer seed match mutated from AGCAC…

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
8_T7-PH-mScarlet3

Plasmid

#225935

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the PH membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

PH-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9A-HA

Plasmid

#209230

Purpose

Mammalian expression of ATP9A

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9A (ATP9A Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

Plasmid

#216170

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

Plasmid

#216172

Purpose

Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- Control- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-b(E184Q)-HA

Plasmid

#209264

Purpose

Mammalian expression of ATP11C isoform b mutant E184Q

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Use

Retroviral

Tags

HA

Mutation

E184Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C-c-HA

Plasmid

#209222

Purpose

Mammalian expression of ATP11C isoform c

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform c (ATP11C Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C-b-HA

Plasmid

#209221

Purpose

Mammalian expression of ATP11C isoform b

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207-EBM1-2mut_K

Plasmid

#146756

Purpose

Bacterial Expression of HsSmg6_1-207-EBM1-2mut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207-EBM1-2mut (SMG6 Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207-EBM1mut_K

Plasmid

#146757

Purpose

Bacterial Expression of HsSmg6_1-207-EBM1mut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207-EBM1mut (SMG6 Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207-EBM2mut_K

Plasmid

#146758

Purpose

Bacterial Expression of HsSmg6_1-207-EBM2mut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207-EBM2mut (SMG6 Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsC2orf68_K

Plasmid

#146760

Purpose

Mammalian Expression of HsC2orf68

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsC2orf68 (C2orf68 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsR3HCC1_39-480_K

Plasmid

#146764

Purpose

Mammalian Expression of HsR3HCC1_39-480

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsR3HCC1_39-480 (R3HCC1 Human)

Expression

Mammalian

Mutation

sequence extracted from BC050572.1; 39-480 trunca…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTDRD3_K

Plasmid

#146768

Purpose

Mammalian Expression of HsTDRD3

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsTDRD3 (TDRD3 Human)

Expression

Mammalian

Mutation

two non silent mutations H100R and P508H compared…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTDRD3-EBMmut_K

Plasmid

#146769

Purpose

Mammalian Expression of HsTDRD3-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsTDRD3-EBMmut (TDRD3 Human)

Expression

Mammalian

Mutation

two non silent mutations H100R and P508H compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-515_I

Plasmid

#146566

Purpose

Bacterial Expression of HsSmg6_1-515

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-515 (SMG6 Human)

Expression

Bacterial

Mutation

two silent mutations and two non silent mutation …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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