159,557 results

1. P2RY6-Tango

   Plasmid

   #66476

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY6 (P2RY6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. UbC-Halo-SFPQ

   Plasmid

   #166946

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   Promoter

   UbC

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139089

   Purpose

   Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   xCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5 BMPS HK318/9EE-6xHis

   Plasmid

   #204836

   Purpose

   Mammalian expression of recombinant BMPS WT

   Depositor

   Monther Abu-Remaileh

   Article

   Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

   Insert

   CLN5 (CLN5 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   HK318/9EE

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFLAG-CMV2-Hsp27-S15D/S78D/S82D

   Plasmid

   #85188

   Purpose

   Hsp27 expression plasmid

   Depositor

   Ugo Moens

   Insert

   Hsp27 (HSPB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S15D/S78D/S82D, phosphomimicking mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

   Plasmid

   #100157

   Purpose

   Expresses MUS81

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   MUS81 (MUS81 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA6-N-Myc-SCAP

   Plasmid

   #134285

   Purpose

   Expresses Myc-tagged SCAP in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SCAP (Scap, Scap Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-3c-rhVTN-N

   Plasmid

   #30225

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 Apr 10. ():.

   Insert

   rhVitronectin with N terminus cleavage (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids: 20*-62

   Promoter

   Available since

   May 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hHS1

   Plasmid

   #159169

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge