162,651 results

1. pWN_U6-B2M-miniGag-Cas9

   Plasmid

   #228958

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFC0984

   Plasmid

   #231992

   Purpose

   CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Inserts

   dRfxCas13d
   crRNA with 2xBsaI spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and pTet

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-puro-CMV-VC3AI

   Plasmid

   #78907

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   VC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Expression

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherryC1 VAP-B

   Plasmid

   #226409

   Purpose

   Expression of human VAP-B fused to mCherry in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   VAP-B (VAPB Human)
   

   Use

   Tags

   HA, T7-Xpress tag, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. IDG_KCNA6_OE_1

   Plasmid

   #161643

   Purpose

   Overexpresses KCNA6

   Depositor

   Mike McManus

   Insert

   KCNA6 (KCNA6 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. VDR-NanoLuc Fusion Vector

   Plasmid

   #236958

   Purpose

   ExpressVDR-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   VDR (VDR Human)
   

   Use

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCDH-3_Flag-YTHDF2-RNA-MUT (nonsense mutation of sgYTHDF2-1)

   Plasmid

   #232948

   Purpose

   Expresses the RNA-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

   Depositor

   Jianjun Chen

   Article

   Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

   Insert

   YTHDF2-RNA-MUT (YTHDF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   RNA binding mutation (K416A+R527A), synonymous mu…

   Promoter

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-3_Flag-YTHDF2-D-MUT (nonsense mutation of sgYTHDF2-1)

   Plasmid

   #232947

   Purpose

   Expresses the domain-mutant YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

   Depositor

   Jianjun Chen

   Article

   Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

   Insert

   YTHDF2-D-MUT (YTHDF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   5 site mutation (Y418A+D422A+W432A+W486A+W491A), …

   Promoter

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-3_Flag-YTHDF2 (nonsense mutation of sgYTHDF2-1)

   Plasmid

   #232945

   Purpose

   Expresses YTHDF2 with synonymous mutations rendering it resistant to sgYTHDF2-1-mediated knockdown

   Depositor

   Jianjun Chen

   Article

   Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   synonymous mutation

   Promoter

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-3_Flag-YTHDF2-m(6)A-MUT (nonsense mutation of sgYTHDF2-1)

    Plasmid

    #232946

    Purpose

    Expresses the m6A-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-m(6)A-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    2 site mutation (W432A+W486A), synonymous mutation

    Promoter

    Available since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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