Addgene

We narrowed to 6,446 results for: poly

Showing: 2851 - 2880 of 6446 results

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pXD80EN3-Elen0507-Cadh-noAccs(ElA2)

Plasmid

#225311

Purpose

Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2)

Plasmid

#225312

Purpose

Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)

Plasmid

#225298

Purpose

Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pZH60 pLIB-spt5

Plasmid

#231648

Purpose

express S.cerevisiae spt5 in insect cells

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

spt5 (SPT5 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB602-MpPHOT-3xFLAG

Plasmid

#228474

Purpose

MpPHOT-3xFLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT-3xFLAG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pZB260_pT7-STR-H3U3

Plasmid

#228506

Purpose

Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

HIS3/URA3 operon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBSsgRNA

Plasmid

#224867

Purpose

Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAM284

Plasmid

#226122

Purpose

Expression of cdc48 E588Q

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 E588Q (CDC48 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

E588Q

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM290

Plasmid

#226127

Purpose

Expression of His6-GST-3C-Npl4

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 (HIS6 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM289

Plasmid

#226126

Purpose

Expression of Npl4 D460K/Y461A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 D460K/Y461A (NPL4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

D460K/Y461A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM358

Plasmid

#226138

Purpose

Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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paM291

Plasmid

#226128

Purpose

Expression of His6-GST-3C-Npl4 T255A/T571A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 T255A/T571A (HIS6 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

T255A/T571A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM288

Plasmid

#226125

Purpose

Expression of Npl4 W252A/R253E

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 W252A/R253E (NPL4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

W252A/R253E

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM285

Plasmid

#226123

Purpose

Expression of cdc48 W561A/Y562A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 W561A/Y562A (CDC48 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

W561A/Y562A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPSt

Plasmid

#203590

Purpose

Base plasmid for Plant STARR-seq terminator libraries.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ248

Plasmid

#217494

Purpose

Mammlian expression of DpFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc guide RNA scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MGS0156_pET29b

Plasmid

#215410

Purpose

Expression construct for MGS0156 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MGS0156

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ234

Plasmid

#217492

Purpose

Mammlian expression of DpFnuc for genome editing

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12720

Plasmid

#212704

Purpose

gRNA targeting to the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the gene 4HPPD locus

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN100

Plasmid

#205073

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 100 amino acids

Promoter

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204786

Purpose

Bacterial expression of Sars Cov 2 MPRO T190I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

T190I

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204784

Purpose

Bacterial expression of Sars Cov 2 MPRO Q189K mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

Q189K

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204783

Purpose

Bacterial expression of Sars Cov 2 MPRO P132H mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

P132H

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204782

Purpose

Bacterial expression of Sars Cov 2 MPRO N142S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

N142S

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204779

Purpose

Bacterial expression of Sars Cov 2 MPRO M49T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

M49T

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204777

Purpose

Bacterial expression of Sars Cov 2 MPRO M49I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

M49I

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204776

Purpose

Bacterial expression of Sars Cov 2 MPRO G143S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

G143S

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204774

Purpose

Bacterial expression of Sars Cov 2 MPRO A191V mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

A191V

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204773

Purpose

Bacterial expression of Sars Cov 2 MPRO A193T mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

A193T

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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