We narrowed to 5,473 results for: KRAS

1. pLVX-RT007-GCN4scFV-VB

   Plasmid

   #163387

   Purpose

   2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV_AA089

   Plasmid

   #216105

   Purpose

   gRNA expression for Cas12a (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFnCas12a_NT

   Plasmid

   #169838

   Purpose

   Expresses FnCas12a and guide RNA in Bacteria

   Depositor

   John van der Oost

   Article

   Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

   Insert

   FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pXynA

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TPI in pCW57_tTA_Blast

   Plasmid

   #154209

   Purpose

   Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   TPI1 (TPI1 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Promoter

   TRE repeats (dox off)

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAXEF-Rap1-E63(GTP)

   Plasmid

   #32698

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Expression

   Mammalian

   Mutation

   E63: Consitutively GTP-bound

   Promoter

   EF promoter

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9624-BEAR-cloning

   Plasmid

   #162986

   Purpose

   BEAR target cloning plasmid with split EGFP

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS1ClacZ

   Plasmid

   #55171

   Purpose

   lacZ-reporter vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

8. 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

   Plasmid

   #174424

   Purpose

   Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(Thrombin)EGFP(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

   Plasmid

   #177183

   Purpose

   plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   sgRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-SAMTOR (D190A)

    Plasmid

    #100515

    Purpose

    Expresses HA tagged SAMTOR D190A mutant in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D190A

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_836

    Plasmid

    #216088

    Purpose

    Cas9 [Sp] knockout targeting CD55, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD55 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172505

    Purpose

    Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-mCherry-C1-GtACR1

    Plasmid

    #85464

    Purpose

    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR1 gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+enhancer)

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pN1-Lck-GCaMP2

    Plasmid

    #24794

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

    Insert

    Lck-GCaMP2
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-mCherry-C1-GtACR2

    Plasmid

    #85463

    Purpose

    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR2 gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+enhancer)

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

    Plasmid

    #172515

    Purpose

    Encodes HvsCas7-11 mammalian expression casette in AAV backbone

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV EFS-HvsCas7-11 for dual vector
    

    Use

    AAV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SaCas9 TadDE

    Plasmid

    #193846

    Purpose

    Express TadDE (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE with SaCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT9675-CBE

    Plasmid

    #163007

    Purpose

    plasmid expressing CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SEH1L in pRK5

    Plasmid

    #154216

    Purpose

    Untagged SEH1L cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    SEH1L (SEH1L Human)
    

    Tags

    NONE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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