We narrowed to 17,522 results for: She

1. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

   Plasmid

   #159576

   Purpose

   Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2FPP

   Plasmid

   #203758

   Purpose

   Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   HRas c-term (HRAS Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 1 kb prom. + Enhancer CD274

   Plasmid

   #107005

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   N.B. There are two published SNPs in this region …

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE_HRASV12_Hyg

   Plasmid

   #195143

   Purpose

   HRas G12V overexpression vector with hygroymycin resistance

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   hRAS (HRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEAT8-137 M1V

   Plasmid

   #173807

   Purpose

   Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

   Depositor

   Anne Gershenson

   Article

   Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

   Insert

   SERPiNA1 (SERPINA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E1M Otherwise this plasmid encodes the most commo…

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5-Flag-Ago2

   Plasmid

   #72207

   Purpose

   Mammalian expression of full-length human Ago2

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   argonaute RISC catalytic component 2 (AGO2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221 UBR5 ∆PABC

   Plasmid

   #81064

   Purpose

   Gateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Y2402A and K2415A (bp t7204g, a7205c, t7206c, a72…

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-ProDOL

   Plasmid

   #206866

   Purpose

   Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag

   Depositor

   Dirk-Peter Herten

   Article

   Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

   Insert

   ProDOL
   

   Use

   Retroviral

   Tags

   HaloTag, His-Tag, SNAP-tag, and eGFP

   Expression

   Mammalian

   Mutation

   Contains Lyn amino acids 1-16

   Promoter

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28(+)-NudC

    Plasmid

    #175022

    Purpose

    Purification of NudC enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    nudC
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-HTR6-SNAPf-TRIP

    Plasmid

    #189617

    Purpose

    Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6-TRIP_alpha
    

    Use

    AAV

    Tags

    alphaTag in the N-term of HTR6, SNAP-tag in the C…

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-NSD3-short W284A

    Plasmid

    #72551

    Purpose

    expresses 3*FLAG tagged human NSD3-short with W284A mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    W284A

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. TP53-KO_gRNA_2

    Plasmid

    #195131

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 2 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXB6 (human) FLAG pMSCV

    Plasmid

    #8534

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pSUPER-retro-puro-Smad2

    Plasmid

    #89827

    Purpose

    Knockdown human Smad2 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    SMAD2 shRNA (SMAD2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. integrin alpha9 / alpha5 pcDNA1 neo

    Plasmid

    #13588

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-NSD3-short del(1-100)

    Plasmid

    #72547

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    1-100 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. integrin alpha9 / alpha4 pcDNA1 neo

    Plasmid

    #13587

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 4 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. integrin alpha9 / alpha2 pcDNA1 neo

    Plasmid

    #13586

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 2 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-BRAF-WT-IRES-mCherry

    Plasmid

    #221027

    Purpose

    Fluorescent reporter for expressing a segment of wild type BRAF

    Depositor

    Mohamed Fareh

    Article

    Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

    Insert

    BRAF wildtype (BRAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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