We narrowed to 7,125 results for: ef1a

1. pLV V5-Sun1

   Plasmid

   #175113

   Purpose

   Lentiviral expression of V5-tagged mouse Sun1

   Depositor

   Larry Gerace

   Insert

   Sun1 (Sun1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV Nup88-V5

   Plasmid

   #175117

   Purpose

   Lentiviral expression of mouse Nup88-V5

   Depositor

   Larry Gerace

   Insert

   Nup88 (Nup88 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV Rbmxl1-V5

   Plasmid

   #175124

   Purpose

   Lentiviral expression of mouse Rbmxl1-V5

   Depositor

   Larry Gerace

   Insert

   Rbmxl1 (Rbmxl1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Puro_hCOL4A4-3FLAG

   Plasmid

   #198101

   Purpose

   lentiviral expression of hCOL4A4 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A4 (COL4A4 Human)
   

   Use

   Lentiviral

   Tags

   3FLAG

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro_hCOL4A3

   Plasmid

   #198098

   Purpose

   lentiviral expression of hCOL4A3 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A3 (COL4A3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ELL3 Streptag

   Plasmid

   #127282

   Purpose

   Expresses mouse ELL3 with streptactin tag; does it degrade Myc?

   Depositor

   Christine Milcarek

   Insert

   ELL3 (NM_145973) (ELL3 Mouse)
   

   Use

   Tags

   Twin strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-Puro_LPAR_AKAR-WT

   Plasmid

   #125206

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

   Depositor

   Eric Davis

   Insert

   LPAR_AKAR-WT
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV V5-Senp2

    Plasmid

    #175118

    Purpose

    Lentiviral expression of V5-tagged mouse Senp2

    Depositor

    Larry Gerace

    Insert

    Senp2 (Senp2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-puro-N-3HA-SREBP1-C-Flag

    Plasmid

    #134286

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. gGBEv6.3-SpG

    Plasmid

    #202630

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpG(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. gCBEv2

    Plasmid

    #220617

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. gTBEv3

    Plasmid

    #220619

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55647

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1a

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiU6-LacZ-GFP-Puro (BB)

    Plasmid

    #170459

    Purpose

    For cloning gRNA block

    Depositor

    Yonglun Luo

    Article

    Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

    Insert

    hU6-BB-LacZ and EF1A-EGFP-2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 for gRNA and EF1A for EGFP-2A-Puro

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS191

    Plasmid

    #197140

    Purpose

    scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    EF1a

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

    Plasmid

    #134252

    Purpose

    Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 179-219, and mutated coding s…

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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