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pBEL1245

Plasmid

#155139

Purpose

Expresses MlaF in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaF

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1246

Plasmid

#155141

Purpose

Expresses MlaB in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaB

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Crimson)

Plasmid

#140154

Purpose

Expresses a vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) AcAc

Plasmid

#111332

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(AcAc)

Plasmid

#111327

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-iGFP-YPT52

Plasmid

#105279

Purpose

Plasmid for integration of iGFP-Ypt52 at TRP1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt52 (YPT52 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB3647

Plasmid

#80981

Purpose

Used for abrB3647(CAC3647) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB3647(CAC3647)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB1941

Plasmid

#80980

Purpose

Used for abrB1941(CAC1941) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB1941(CAC1941)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB0310

Plasmid

#80979

Purpose

Used for abrB0310(CAC0310) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB0310(CAC0310)

Expression

Bacterial

Promoter

thl (cac2873)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pALBRSF12

Plasmid

#46376

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE intein, inactive

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A, C+1A

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSABAD25-74

Plasmid

#46375

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

TvoVMA intein

Tags

H6-GB1 and SH3

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a207-479.wt

Plasmid

#17516

Depositor

David McKay

Article

Wang et al Biophys J. 2008 Jan 1. 94(1):L01-3.

Insert

B. subtilis DEAD-box helicase YxiN second domain

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 1-206

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pSF1389

Plasmid

#104962

Purpose

Bacterial expression plasmid of bdSENP1 protease

Depositor

Dirk Görlich

Article

Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

Insert

SENP1

Tags

His14 tag and Tev protease cleavage site

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_IRES_puro2b

Plasmid

#21042

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry_a_tubulin_IRES_puro2

Plasmid

#21043

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-PIK1

Plasmid

#105260

Purpose

Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Pik1 (PIK1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl

Plasmid

#90485

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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