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pTORGm43GWY

Plasmid

#112906

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

Plasmid

#211861

Purpose

Nuclear-localized E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CC

Plasmid

#105116

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2/mVenus

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB8c-GFP

Plasmid

#35362

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGWT35S-OEP7-mCherry

Plasmid

#182587

Purpose

Transient expression of OEP7-mCherry in plant cell (Outer envelope membrane of plastid/chloroplast)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

OEP7

Tags

mCherry

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHN1_lacUV5-mNG-PDT

Plasmid

#165583

Purpose

Expresses mNeonGreen and C-terminally fused PDT

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen-PDT

Tags

Protein degradation tag

Expression

Bacterial

Promoter

Ptrc

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSG28-J23151-AraC

Plasmid

#102453

Purpose

Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

AraC (araC )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pUdO1c

Plasmid

#210230

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGT-Ah5

Plasmid

#122572

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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