We narrowed to 2,368 results for: pcas9

1. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ922

   Plasmid

   #78312

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-EGFP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO5.sgRNA.EFS.PAC

   Plasmid

   #57825

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e-N108Q

   Plasmid

   #194157

   Purpose

   Mammalian expression of ABE8e-N108Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCTRE-CD4

   Plasmid

   #114010

   Purpose

   Dox-inducible Cas9 expression with a CD4 selection marker

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMGS7 (AAVS1sgRNA)

   Plasmid

   #126582

   Purpose

   A CRISPR/Cas9 construct to target the AAVS1 locus in humans

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AAVS1 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-Amp_CRISPIE donor B5

   Plasmid

   #172846

   Purpose

   CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cre-responsive Cas9 expressing transposon library vector

   Plasmid

   #164138

   Purpose

   Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6, EF-1a

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHPHS800

    Plasmid

    #228240

    Purpose

    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

    Depositor

    Arvind Rasi Subramaniam

    Article

    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

    Insert

    Cas9
    

    Use

    Tags

    Hygromycin phosphotransferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-NRCH-PE2max-BSD

    Plasmid

    #191103

    Purpose

    Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    NRCH-PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9-NRCH

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. YEE-BE4max

    Plasmid

    #138157

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YEE)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Td-CGBE

    Plasmid

    #196599

    Purpose

    Mammalian expression of Td-CGBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L); S. pyogenes Cas9 (D10A)

    Promoter

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBR1.cas9-NT

    Plasmid

    #197871

    Purpose

    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

    Depositor

    Baojun Wang

    Article

    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

    Inserts

    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR-cBEST

    Plasmid

    #125689

    Purpose

    C to T base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Insert

    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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