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tdEos-VE-Cadherin-N-10
Plasmid#57687PurposeLocalization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVE-Cadherin (CDH5 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
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SDCBPA-c010
Plasmid#175482PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-6a-Ataxin3Q22-UIM*
Plasmid#22128DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsFlagExpressionMammalianMutationUIM 1,2,3 SA-DGPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
IDG_CLIC2_OE_1
Plasmid#161642PurposeOverexpresses CLIC2DepositorInsertCLIC2 (CLIC2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E028 Hs.CNKSR1-nostop
Plasmid#70312PurposeGateway ORF clone of human CNKSR1 [NM_001297647.1] without stop codon (for C-terminal fusions)DepositorInsertCNKSR1 (CNKSR1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro
Plasmid#121175PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell linesDepositorInsertGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRCMV 14-3-3 sigma
Plasmid#16552DepositorInsert14-3-3 sigma (SFN Human)
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
R777-E108 Hs.INSRR-nostop
Plasmid#70392PurposeGateway ORF clone of human INSRR [NM_014215.2] without stop codon (for C-terminal fusions)DepositorInsertINSRR (INSRR Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-R1398Q
Plasmid#29398DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationArg1398GlnPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-T1343G
Plasmid#29400DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationThr1343GlyPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
dClover2-Tubulin-C-18
Plasmid#56376PurposeLocalization: Microtubules, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.DepositorInsertTubulin (TUBA1B Human)
UseTagsdClover2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pcDNA3.1-CD8bM-3
Plasmid#40080DepositorInsertCD8bM-3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
4QQN
Plasmid#61951PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHRMT1L3:APC035-H01:C21507 (PRMT3 Human)
UseTags6xHis-Thrombin and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-EIF2C3-C-18
Plasmid#54078PurposeLocalization: Cytoplasm, Excitation: 487, Emission: 509DepositorInsertEIF2C3 (AGO3 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mAmetrine-Vimentin-7
Plasmid#56551PurposeLocalization: Intermediate Filaments, Excitation: 406, Emission: 526DepositorInsertVimentin (VIM Human)
UseTagsmAmetrineExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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R777-E018 Hs.CCND1-nostop
Plasmid#70302PurposeGateway ORF clone of human CCND1 [NM_053056.2] without stop codon (for C-terminal fusions)DepositorInsertCCND1 (CCND1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUDT17 (NUDT17A-c014)
Plasmid#98224PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNUDT17A (NUDT17 Human)
UseBaculovirusTagsHis6-ZBasic-TEVExpressionMutationpfam00293:NUDIX 93-235Promoterpolyhedrin promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hMALAT1 deletion 11 PARP1 binding sites
Plasmid#159327PurposeMammalian cell expression of human MALAT1 with deleted PARP1 binding sites. Contains CopGFP marker.DepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion 11 PARP1 binding sitesPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E069 Hs.EXOC2
Plasmid#70353PurposeGateway ORF clone of human EXOC2 [NM_018303.5] with stop codon (for native or N-terminal fusions)DepositorInsertEXOC2 (EXOC2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-Alpha-Actinin-19
Plasmid#57260PurposeLocalization: Alpha Actinin, Excitation: 503, Emission: 518DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsDronpaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
R777-E052 Hs.ECT2-nostop
Plasmid#70336PurposeGateway ORF clone of human ECT2 [NM_018098.5] without stop codon (for C-terminal fusions)DepositorInsertECT2 (ECT2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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mEos4a-CenpA-C-22
Plasmid#62772PurposeLocalization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581DepositorInsertCenpA (CENPA Human)
UseTagsmEos4aExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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mEmerald EFHC2-N-18
Plasmid#54077PurposeLocalization: EF-Hand Domain , Excitation: 487, Emission: 509DepositorInsertEFHC2 (EFHC2 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-ALK1-C-18
Plasmid#53980PurposeLocalization: Membrane Receptor, Excitation: 487, Emission: 509DepositorInsertALK1 (ACVRL1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagRFP-T-CENPB-N-22
Plasmid#58004PurposeLocalization: Nucleus/Centromeres, Excitation: 555, Emission: 584DepositorInsertCENPB (CENPB Human)
UseTagsmTagRFPExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
4JDS
Plasmid#60081PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD7:DCC011-H01:C212434 (SETD7 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-ATG14-C-18
Plasmid#54006PurposeLocalization: Membrane Receptor, Excitation: 487, Emission: 509DepositorInsertATG14 (ATG14 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET11b-MIF Y99A
Plasmid#149440PurposeExpression of MIF Y99A in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99APromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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mCerulean-Vimentin-7
Plasmid#55395PurposeLocalization: Intermediate Filaments, Excitation: 433, Emission: 475DepositorInsertVimentin (VIM Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
UBH12 (3CLZ)
Plasmid#25220PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubh12.414.617:B9 (UHRF1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-ARP3-C-12
Plasmid#57582PurposeLocalization: Actin Related Protein, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertARP3 (ACTR3 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
RRAGC
Plasmid#25335PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRRAGC:S60-I237 (RRAGC Human)
UseTagsHisExpressionBacterialMutationContains amino acids S60-I237PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdEos-Desmocollin1-N-18
Plasmid#57608PurposeLocalization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertDesmocollin1 (DSC1 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-ATG13-C-18
Plasmid#54004PurposeLocalization: Membrane Receptor, Excitation: 487, Emission: 509DepositorInsertATG13 (ATG13 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDk4-luciferase fragment 1
Plasmid#86660PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated from 880 -1 to -213PromoterCDK8Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c020
Plasmid#139757PurposeFor protein expression/crystallography (PDB 5QS0)DepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationContains only amino acids E41-N211PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human ZP3 (JD#184)
Plasmid#14633DepositorInsertHuman ZP3 (cDNA) (ZP3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
R777-E031 Hs.CYTH2
Plasmid#70315PurposeGateway ORF clone of human CYTH2 [NM_017457.5] with stop codon (for native or N-terminal fusions)DepositorInsertCYTH2 (CYTH2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSPDEF-donor
Plasmid#113828PurposeCRISPR donor plasmid to tag human transcription factor SPDEF with GFPDepositorInsertSPDEF homology arms flanking EGFP-IRES-Neo cassette (SPDEF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKO2-Dectin1A-C-10
Plasmid#57869PurposeLocalization: Membrane, Excitation: 551, Emission: 565DepositorInsertDectin1A (CLEC7A Human)
UseTagsmKO2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c026
Plasmid#139763PurposeProtein expression/GST pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
