Addgene

We narrowed to 5,096 results for: PEP

Showing: 1921 - 1940 of 5096 results

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pHH0103_SIAH2_Sina

Plasmid

#109813

Purpose

Protein expression and purification of SIAH2_Sina

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SIAH2_Sina (SIAH2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Use

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-mCherry

Plasmid

#193864

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Use

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-mCherrySTOP

Plasmid

#193866

Purpose

E. coli expression vector for N-ter GFP11 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Use

Tags

6xHis and GFP11

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVIM-BC2T

Plasmid

#108967

Purpose

expresses vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

Vimentin

Use

Tags

BC2T

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLAT_Trypsin

Plasmid

#110039

Purpose

Protein expression and purification of PLAT_Trypsin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLAT_Trypsin (PLAT Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAH_14-3-3

Plasmid

#109988

Purpose

Protein expression and purification of YWHAH_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAH_14-3-3 (YWHAH Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN2_Profilin

Plasmid

#110007

Purpose

Protein expression and purification of PFN2_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN2_Profilin (PFN2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET14_GFP5_Encap

Plasmid

#86408

Purpose

Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 5aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_XRCC4_XRCC4

Plasmid

#110047

Purpose

Protein expression and purification of XRCC4_XRCC4

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

XRCC4_XRCC4 (XRCC4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pZA-mCherry-R5

Plasmid

#106258

Purpose

Expresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetO

Depositor

Urartu Seker

Article

Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

Insert

mCherry

Use

Tags

Added silaffin R5 peptide to C terminus of mCherr…

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only
pHH0103_RPA1_RepA-N

Plasmid

#110032

Purpose

Protein expression and purification of RPA1_RepA-N

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RPA1_RepA-N (RPA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R375

Plasmid

#138245

Purpose

Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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H2B-CoilE-mEm

Plasmid

#181992

Purpose

CoilE genetically encoded tag on H2B-mEmerald, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

H2B-CoilE-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1

Plasmid

#62600

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (FPR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only
pKB237

Plasmid

#170030

Purpose

Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL19 (CCL19 Escherichia coli, Synthetic, Human)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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BB-NIC-II-HisN-TET12

Plasmid

#45496

Depositor

Roman Jerala

Article

Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

Insert

TET12

Use

Synthetic Biology

Tags

6xHistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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HisSumoOphP

Plasmid

#189672

Purpose

HisSUMO tagged OphP to express OphP in pichia pastorias.

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

HisSumoOphP

Use

Tags

HisSumo tagged

Expression

Yeast

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Use

Tags

GST and thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRPK2_Pkinase

Plasmid

#110026

Purpose

Protein expression and purification of SRPK2_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SRPK2_Pkinase (SRPK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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