We narrowed to 6,931 results for: crispr cas9 plasmids

1. pMLS254

   Plasmid

   #73721

   Purpose

   SapTrap Halotag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Halotag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONOR 6colors

   Plasmid

   #229974

   Purpose

   bigMamAct assembled plasmid for 6colors cellular labelling

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. psLIB CMV

   Plasmid

   #229953

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psLIB U6

   Plasmid

   #229956

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. psLIB EF1alpha

   Plasmid

   #229954

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psLIB TRE

   Plasmid

   #229955

   Purpose

   bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMLS292

   Plasmid

   #73725

   Purpose

   SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   2xNLS-mCherry + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

   Plasmid

   #154429

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZBED9.1.1-gDNA

   Plasmid

   #175854

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZBED9-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZBED9.1.2-gDNA

    Plasmid

    #175853

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZBED9-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDX4.1.1-gDNA

    Plasmid

    #175852

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    CDX4-Nickase1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDX4.1.2-gDNA

    Plasmid

    #175851

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    CDX4-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZMAT4.1.1-gDNA

    Plasmid

    #175871

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZMAT4-Nickase1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ZMAT4.1.2-gDNA

    Plasmid

    #175872

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZMAT4-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HEY2.1.1-gDNA

    Plasmid

    #175873

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    HEY2-Nickase1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HEY2.1.2-gDNA

    Plasmid

    #175874

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    HEY2-Nickase2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OVOL2.2.1-gDNA

    Plasmid

    #175876

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OVOL2-Nickase1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OVOL2.2.2-gDNA

    Plasmid

    #175877

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OVOL2-Nicakse2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPHB.1.0-gDNA

    Plasmid

    #132453

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    PHB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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