Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,422 results for: dcas9

Clear keywords
Showing: 1921 - 1940 of 2422 results

  2. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

    Enlarge

  3. LLP789_αGCN4-DNMT3A-CO

    Plasmid
    #211783
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    aGCN4-DNMT3A
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    211783-plasmid-map-sequence-id-417155

    Enlarge

  16. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid
    #121750
    Purpose
    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x HA epitope tag + polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121750-plasmid-map-sequence-id-237188

    Enlarge

  17. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid
    #121751
    Purpose
    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    FLAG epitope tag + polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121751-plasmid-map-sequence-id-237190

    Enlarge

  19. KY901: pMVP (L3-L2) P2A-mCherry + polyA

    Plasmid
    #121766
    Purpose
    pMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-mCherry + polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121766-plasmid-map-sequence-id-237260

    Enlarge

Showing: 1921 - 1940 of 2422 results