We narrowed to 4,947 results for: mcs

1. pcDNA3 PARL-FLAG-CT S70D

   Plasmid

   #13623

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Ser 70 to Asp

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCDH-CMV4

   Plasmid

   #72284

   Purpose

   Express gene of interest under CMV promoter. WPRE has been removed to increase the cloning capacity.

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 25, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCDH-EF1-FLPe

   Plasmid

   #72262

   Purpose

   Express FLPe under EF-1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   FLPe
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. control (pGS701)

   Plasmid

   #160037

   Purpose

   pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRLuc-IVT

   Plasmid

   #205148

   Purpose

   IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA

   Depositor

   Igor Ulitsky

   Insert

   Renilla Luciferase
   

   Use

   Luciferase; In-vitro transcription

   Promoter

   T7

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTol2-nbt300 2n-KalNFB-syt2a

   Plasmid

   #196901

   Purpose

   zebrafish expression

   Depositor

   Reinhard Köster

   Insert

   nbt300 2n-KalNFB-syt2a
   

   Use

   Zebrafish expression

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ND6-DdCBE-left side TALE

   Plasmid

   #157842

   Purpose

   expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

   Plasmid

   #199580

   Purpose

   Expresses GCaMP8f in a Cre-dependent manner

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   His-DIO GCaMP8f
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hsyn

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXJ-His-CD276

   Plasmid

   #232662

   Purpose

   transient expresses CD276 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   CD276 (CD276 Human)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAF0033 Ef1a-Pa01-2a-EGFP

    Plasmid

    #193460

    Purpose

    Expression of Pa01 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAF0036 Ef1a-Si74-2a-EGFP

    Plasmid

    #193463

    Purpose

    Expression of Si74 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV_AR-RE_MLPmin_BC1011-luc2

    Plasmid

    #227106

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1011

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 HA E2F1

    Plasmid

    #236431

    Purpose

    transient overexpression of E2F1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F1 (E2F1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 HA E2F3B

    Plasmid

    #236438

    Purpose

    transient overexpression of E2F3B in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3B (E2F3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 HA E2F3A

    Plasmid

    #236437

    Purpose

    transient overexpression of E2F3A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3A (E2F3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG-16-3xFLAG-LaG-2

    Plasmid

    #172765

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-3xFLAG-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ND4-DdCBE- left side TALE

    Plasmid

    #157844

    Purpose

    expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge