We narrowed to 2,516 results for: organization Addgene

1. pPB CAG Otx2-IRES-EGFP

   Plasmid

   #153947

   Purpose

   piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Otx2 (Otx2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-BE3-2X

   Plasmid

   #110835

   Purpose

   CMV expression vector for BE3-2X construct (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-2X
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 tandem NLS sequences in the XTEN linker

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CP3

   Plasmid

   #223335

   Purpose

   Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   Toc64-miniTurboID-EGFP-3HA
   

   Use

   Tags

   miniTurboID-EGFP-3HA

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CP1

   Plasmid

   #223333

   Purpose

   Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   Toc64-TurboID-EGFP-3HA
   

   Use

   Tags

   TurboID-EGFP-3HA

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Halotag-6xHp

   Plasmid

   #214414

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to Halotag

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   6x Spastin aa 43-92 (SPAST Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mApple-1xHp

   Plasmid

   #214409

   Purpose

   Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   Spastin aa 43-92 (SPAST Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p420-3

   Plasmid

   #71257

   Purpose

   luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

   Depositor

   Peter Cockerill

   Article

   Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

   Insert

   3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GM-CSF minimal

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-Puro-TDP-43-Q331K

   Plasmid

   #133761

   Purpose

   Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed glutamine 331 to lysine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR-HOT-P2A-Clover-BlastR

   Plasmid

   #138568

   Purpose

   Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   P2A-clover-BlastR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-Flag-Sintbad

    Plasmid

    #234668

    Purpose

    expresses SINTBAD (TBKBP1) in mammalian cells

    Depositor

    Lienhard Schmitz

    Article

    Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-Puro-TDP-43-M337V

    Plasmid

    #133759

    Purpose

    Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed methionine 337 to valine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-Puro-TDP-43-K263E

    Plasmid

    #133758

    Purpose

    Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed lysine 263 to glutamate.

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid

    #127366

    Purpose

    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9(BB)-2A-tomato SOX2-sgRNA

    Plasmid

    #196190

    Purpose

    Editing human SOX2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid

    #127368

    Purpose

    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid

    #127370

    Purpose

    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid

    #127354

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid

    #127353

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge