We narrowed to 2,540 results for: organization Addgene

1. pLVX-NPC1(P691S)-FLAG

   Plasmid

   #164973

   Purpose

   Lentiviral vector for NPC1 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   P691S

   Promoter

   EF1a

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-NPC1(F503/4A)-FLAG

   Plasmid

   #164974

   Purpose

   Lentiviral vector for NPC1 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F503A and F504A

   Promoter

   EF1a

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB CAG Otx2-IRES-EGFP

   Plasmid

   #153947

   Purpose

   piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Otx2 (Otx2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FAB(PX-LD)

   Plasmid

   #214426

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FKBP-V5
   

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET14b-dabAB2

   Plasmid

   #133013

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/mCherry-pBirA-HA

   Plasmid

   #154458

   Purpose

   expresses mCherry-pBirA-HA/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   mCherry-pBirA-HA
   

   Expression

   Mammalian

   Promoter

   CMV-F, T7 promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFE-hCA

   Plasmid

   #132999

   Purpose

   pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Insert

   hCAII (CA2 Human)
   

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

   Plasmid

   #176400

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   FRB-RGG-GFP-RGG-RGG
   

   Tags

   FRB and GFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-ER-EmEGFP

   Plasmid

   #169000

   Purpose

   Express ER-EmEGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
   

   Use

   Unspecified

   Tags

   EGFP

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p7612 MSCV-P C-FlagHA 16E7 E10K

    Plasmid

    #163303

    Purpose

    Expresses HPV16 E7 E10K

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 E10K
    

    Use

    Retroviral

    Tags

    FlagHA

    Mutation

    E10K

    Promoter

    MSCV LTR

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-BE3-2X

    Plasmid

    #110835

    Purpose

    CMV expression vector for BE3-2X construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-2X
    

    Expression

    Mammalian

    Mutation

    2 tandem NLS sequences in the XTEN linker

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CP3

    Plasmid

    #223335

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-miniTurboID-EGFP-3HA
    

    Tags

    miniTurboID-EGFP-3HA

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CP2

    Plasmid

    #223334

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-EGFP-TurboID-3HA
    

    Tags

    EGFP-TurboID-3HA

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CP1

    Plasmid

    #223333

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-TurboID-EGFP-3HA
    

    Tags

    TurboID-EGFP-3HA

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CP4

    Plasmid

    #223336

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-EGFP-miniTurboID-3HA
    

    Tags

    EGFP-miniTurboID-3HA

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Halotag-6xHp

    Plasmid

    #214414

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to Halotag

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (SPAST Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mApple-1xHp

    Plasmid

    #214409

    Purpose

    Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p420-3

    Plasmid

    #71257

    Purpose

    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

    Insert

    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF minimal

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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