159,617 results

1. TFORF1434

   Plasmid

   #144175

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFKBID (NFKBID Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPOTv6-blast-3Ty:mScarletI:3Ty

   Plasmid

   #201090

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mScaret-I
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-RP-CoV2/RBD-puro

   Plasmid

   #161793

   Purpose

   Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

   Insert

   SARS-CoV-2 RBD
   

   Use

   Transposon

   Tags

   6xHis and dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNSU299_GAL2

   Plasmid

   #166088

   Purpose

   Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   GAL2 (GAL2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCL28-decoder

   Plasmid

   #159450

   Purpose

   Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.

   Depositor

   Joerg Stelling

   Article

   Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

   Insert

   PlexAtet-LexA-ER-B112-tCyc1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PlexAtet

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCL66-decoder

   Plasmid

   #159453

   Purpose

   Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

   Depositor

   Joerg Stelling

   Article

   Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

   Insert

   Pfus1mut-tetR-nls-malE-tCyc1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pfus1mut

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1937

   Plasmid

   #79782

   Purpose

   High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

   Insert

   Ple260
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Ple260 (PAX6 MiniPromoter)

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCAG-eCas9-GFP-U6-gRNA

   Plasmid

   #79145

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

   Depositor

   Jizhong Zou

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-APOBEC1-YTH

   Plasmid

   #131636

   Purpose

   Expresses APOBEC1 fused to the YTH domain

   Depositor

   Kate Meyer

   Article

   Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

   Insert

   APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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