159,909 results

1. pTECH-MmPylRS(IPYE)

   Plasmid

   #104073

   Purpose

   Expression plasmid encoding MmPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmPylRS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT29

   Plasmid

   #138521

   Purpose

   phage cloning

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   phage genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SynaptoTAG2

   Plasmid

   #175275

   Purpose

   AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
   

   Use

   AAV

   Tags

   EGFP and tdTomato (P2A cleavage)

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV TRE3G BCL2L1 Neo

   Plasmid

   #140749

   Purpose

   Inducibly expresses Bcl-xL in mammalian cells

   Depositor

   Scott Dixon

   Article

   Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-CalfluxCTN

   Plasmid

   #190068

   Purpose

   Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

   Depositor

   Carl Johnson

   Article

   Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

   Insert

   CalfluxCTN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-FAST-p10

   Plasmid

   #89152

   Purpose

   Express p10-FAST in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   NBV p10-FAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Neo-Bam

   Plasmid

   #16440

   Purpose

   Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nEFCas9

   Plasmid

   #87115

   Purpose

   SpCas9 expression AAV backbone plasmid under control of nEF promoter

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   nEF-Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-TGFb1

    Plasmid

    #101762

    Purpose

    TGFb1 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    transforming growth factor beta 1 promoter (TGFB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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