178,101 results

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Publication

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HPV16 E6 TCR

Plasmid

#122727

Purpose

Express HPV16 E6 TCR

Depositor

Christian Hinrichs

Publication

Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

Insert

HPV16 E6 TCR

Use

Retroviral

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 62.4

Plasmid

#123123

Purpose

Human expression vector for deltaproteobacteria CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Publication

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5-HA-Ubiquitin-K11R

Plasmid

#121154

Purpose

Mammalian expression of HA tagged ubiquitin K11R

Depositor

Josef Kittler

Publication

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K11 mutated to an R

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiGuide

Plasmid

#117986

Purpose

pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

Depositor

Paul Khavari

Publication

Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

Insert

sgRNA backbone (F+E)

Use

Lentiviral

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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EF1a_ASCL1_P2A_Hygro_Barcode

Plasmid

#120427

Purpose

Barcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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