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Plasmid#63206PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
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pAG416GALL-ccdB-6stop
Plasmid#203511PurposeGateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-ccdB-6stop
Plasmid#203512PurposeGateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(1-611)
Plasmid#39336DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(166-611)
Plasmid#39338DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-pIME2
Plasmid#191789PurposeYeast gene induction with meiotic promoterDepositorInsertKanMX pIME2
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-pSPR3
Plasmid#191788PurposeYeast gene induction with meiotic promoterDepositorInsertKanMX pSPR3
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-pSAE3
Plasmid#191787PurposeYeast gene induction with meiotic promoterDepositorInsertKanMX pSAE3
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-pSPO11
Plasmid#191767PurposeYeast gene induction with meiotic promoterDepositorInsertKanMX pSPO11
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCKan
Plasmid#185862PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS415-GPD-PPK
Plasmid#183941PurposeExpresses E. coli PPK from yeast GPD promoterDepositorInsertPPK (ppk E. coli)
UseTagsExpressionYeastMutationWTPromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(162-611)
Plasmid#39337DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCB301-2μ-HDV
Plasmid#196329PurposeA yeast-Escherichia coli-Agrobacterium shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_36_QUEEN-2m
Plasmid#177708PurposePlasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)DepositorInsertpTEF1-QUEEN2m
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBF3038
Plasmid#26850DepositorInsertCreA recombinase
UseCre/LoxTagsExpressionYeastMutationN/APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-natMX6 (pFB1434)
Plasmid#126050PurposeFor C-terminal tagging with TurboID in yeast including nourseothricin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBF3060
Plasmid#26853DepositorInsertCreA recombinase
UseCre/LoxTagsExpressionYeastMutationN/APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIVu
Plasmid#81089PurposeSingle Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03YEMK
Plasmid#28004DepositorInsertAT1.03YEMK
UseTagsmVenus and mseCFPExpressionYeastMutationM60Y, G131E, K132M, 133KPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKT-iRFP-KAN
Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
UseTagsExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…PromoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIVl
Plasmid#81090PurposeSingle Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIVt
Plasmid#81092PurposeSingle Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SIVh
Plasmid#81091PurposeSingle Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -