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We narrowed to 5,049 results for: golden gate

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Showing: 4851 - 4900 of 5049 results

  9. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235307
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235307-plasmid-map-sequence-id-472801

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  11. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #235304
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    FXN-P2A-mRuby2 (FXN Human)
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235304-plasmid-map-sequence-id-472752

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  12. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235308
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235308-plasmid-map-sequence-id-472753

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  13. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #235305
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    FXN-P2A-mRuby2 (FXN Human)
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235305-plasmid-map-sequence-id-472751

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  33. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  40. pNB1-LOX

    Plasmid
    #154058
    Purpose
    Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    LoxP-flanked GUS and TtNAM-B1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    ZmUbi
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154058-plasmid-map-sequence-id-313109

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  42. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

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  43. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  44. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pJEx (Jungle Express)
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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Showing: 4851 - 4900 of 5049 results