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ZfWnt3:Luc

Plasmid

#192992

Purpose

Plasmid to test the Zebrafish Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-pchD WT

Plasmid

#187973

Purpose

Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

Insert

pchD WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Squash-SAM-sensor 4-2

Plasmid

#177916

Purpose

Expresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB-NEO-PYL

Plasmid

#114393

Purpose

For PYL expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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4E1H.95_LUMABS

Plasmid

#155208

Purpose

Expresses 4E1H.95_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY921

Plasmid

#135092

Purpose

Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

Depositor

Yishi Jin

Article

Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

Insert

Pacr-2

Tags

GFP and Synthetic Intron

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-dabAB2

Plasmid

#133012

Purpose

pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 C351A

Plasmid

#133005

Purpose

pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

C351A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-baDAB

Plasmid

#133003

Purpose

pFE carrying the badabA and badabB genes

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

badabB
vcdabA

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ rat MYO1D

Plasmid

#118419

Purpose

For mammalian expression of rat myosin 1D

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

rat myosin 1D (Myo1d Rat)

Expression

Mammalian

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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