We narrowed to 2,540 results for: organization Addgene

1. pLVX-Puro-TDP-43-Q331K

   Plasmid

   #133761

   Purpose

   Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed glutamine 331 to lysine.

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Puro-TDP-43-A315T

   Plasmid

   #133755

   Purpose

   Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed alanine 315 to threonine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. R4pGWB601_UBQ10p-Turbo-NES-YFP

   Plasmid

   #127366

   Purpose

   Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Plant

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Arabidopsis UBQ10 promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. R4pGWB601_UBQ10p-Turbo-YFP-NLS

   Plasmid

   #127368

   Purpose

   Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Arabidopsis UBQ10 promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-Puro-TDP-43-M337V

   Plasmid

   #133759

   Purpose

   Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed methionine 337 to valine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

   Plasmid

   #127370

   Purpose

   Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Arabidopsis UBQ10 promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-Puro-TDP-43-K263E

   Plasmid

   #133758

   Purpose

   Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed lysine 263 to glutamate.

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Flag-Sintbad

   Plasmid

   #234668

   Purpose

   expresses SINTBAD (TBKBP1) in mammalian cells

   Depositor

   Lienhard Schmitz

   Article

   Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

   Insert

   SINTBAD (TBKBP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

   Plasmid

   #127354

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid

    #127353

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid

    #127355

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LAMP1-mCherry-FRB*

    Plasmid

    #207145

    Purpose

    Lysosome-targeted rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    FRB* (T2098L mutant of FKBP-rapamycin binding dom…

    Expression

    Mammalian

    Mutation

    Silent mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Puro-TDP-43-A90V

    Plasmid

    #133754

    Purpose

    Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed alanine 90 to valine

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-Puro-TDP-43-P112H

    Plasmid

    #133760

    Purpose

    Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed proline 112 to histidine.

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-Puro-TDP-43-D169G

    Plasmid

    #133757

    Purpose

    Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed aspartate 169 to glycine.

    Promoter

    CMV

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

    Plasmid

    #127790

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IP-EGFP-LOV2-OMP25

    Plasmid

    #233346

    Purpose

    Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus is deleted and N538E

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP12-CB2mutx2-mTagBFP2-HAx2

    Plasmid

    #207147

    Purpose

    Tandem mutant calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Mutation

    E40Q, E87Q, E131Q, E175Q, E219Q

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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