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Showing: 1941 - 1960 of 2972 results

  2. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Publication
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  3. pME-mCherry-PA-Rac1

    Plasmid
    #169042
    Purpose
    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
    Depositor
    Paola Arlotta
    Publication
    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d
    Insert
    PA-Rac-1 (RAC1 Human, Avena sativa (oat))
    Use
    Gateway entry cloning vector
    Tags
    6X His and Fused with mCherry
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169042-plasmid-map-sequence-id-341784

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  4. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  6. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  7. PF3D7_1012200-COMP-blac-flag-his

    Plasmid
    #111017
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    rhoptry associated adhesin (PF3D7_1012200 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111017-plasmid-map-sequence-id-224584

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  8. ZM2

    Plasmid
    #32784
    Depositor
    George Cross
    Insert
    T7rnap
    Use
    Cre/Lox; Floxed targeting vector for trypanosome
    Available since
    Dec. 13, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pJBL7042

    Plasmid
    #167213
    Purpose
    overexpression of MerR in E. coli for cell-free mercury sensing
    Depositor
    Julius Lucks
    Insert
    MerR
    Expression
    Bacterial
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167213-plasmid-map-sequence-id-330998

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  11. pJBL7047

    Plasmid
    #167217
    Purpose
    overexpression of CadR in E. coli for cell-free cadmium sensing
    Depositor
    Julius Lucks
    Insert
    CadR
    Expression
    Bacterial
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167217-plasmid-map-sequence-id-331011

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  15. pJBL7043

    Plasmid
    #167214
    Purpose
    overexpression of ArsR in E. coli for cell-free arsenic sensing
    Depositor
    Julius Lucks
    Insert
    ArsR EP3
    Expression
    Bacterial
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167214-plasmid-map-sequence-id-331005

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Showing: 1941 - 1960 of 2972 results