We narrowed to 24,452 results for: nes

1. pENTR1A hu_ncOGT WT (open N-term)

   Plasmid

   #155197

   Purpose

   Entry vector encoding non-tagged O-GlcNAc Transferase with open N-terminus

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   WT OGT with no start codon, in-frame with gateway…

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123338

   Purpose

   Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   DAKAP-Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1, Venus, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

   Plasmid

   #123346

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-CKAR
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

   Plasmid

   #123339

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-GFP-GFP-FLARE-AKAR

   Plasmid

   #123332

   Purpose

   Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   GFP-GFP-FLARE-AKAR
   

   Use

   Tags

   6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123337

   Purpose

   Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Lyn-Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, Ve…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-Venus-cpVenus-FLARE-CKAR

   Plasmid

   #123343

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiGuideFB-Puro-A

   Plasmid

   #192506

   Purpose

   For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

   Insert

   hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCK669

   Plasmid

   #192638

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dxCas9-NG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   SoxS has R93A and S101A mutations and dxCas9-NG h…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiGuideFB-Puro-B

    Plasmid

    #192507

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge