We narrowed to 53,286 results for: SON

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pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

Plasmid

#209292

Purpose

Expresses R2Bm-Cas9(H840A) fusion in E. coli

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, SpCas9(H840A), StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Integrin-Beta1-N-18

Plasmid

#55423

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Alpha-5-Integrin-12

Plasmid

#55401

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Alpha-5 Integrin (ITGA5 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
mIFP12-Rab4a-7

Plasmid

#56261

Purpose

Localization: Ras GTPase, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Rab4a (RAB4A Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

Plasmid

#154838

Purpose

Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

synthetic guide::∆HYGR::unc-54 3' UTR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

∆HYGR is promoterless and encodes aa 60-341

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMS4 (Empty insertion vector with SEC)

Plasmid

#154837

Purpose

Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

SEC

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

sqt-1(e1350)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PCNA-19-SV40NLS-4

Plasmid

#55437

Purpose

Localization: Nucleus, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pEJS663-pMCSG7-AcrIIC4Hpa

Plasmid

#113440

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Tags

6xHis

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS664-pMCSG7-AcrIIC5Smu

Plasmid

#113441

Purpose

Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR with N-terminal 6xHis and TEV site

Tags

6xHis

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mIFP-PDHA1-N-10

Plasmid

#56236

Purpose

Localization: Mitochondrial Matrix, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

PDHA1 (PDHA1 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-H2B-C-10

Plasmid

#56254

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Tags

mIFP12

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Endo-14

Plasmid

#56250

Purpose

Localization: Endosomes, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Endo (RHOB Human)

Tags

Myc and mIFP12

Expression

Mammalian

Mutation

Deleted amino acids 1-4

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Endo-14

Plasmid

#56219

Purpose

Localization: Endosomes, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Endo (RHOB Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCerulean3-EB3-7

Plasmid

#55416

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

Plasmid

#113437

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV mouse Vav1DN IRES GFP

Plasmid

#107092

Purpose

Mammalian expression retroviral vector

Depositor

Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 dominant negative cDNA (Vav1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

AA 1-186

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEE044

Plasmid

#176801

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

413

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_04

Plasmid

#158105

Purpose

CaV1.3 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1D (uc003dgu.5)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA

Plasmid

#117407

Purpose

NmeCas9 sgRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

NmeCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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