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pCB301-2μ-HDV

Plasmid

#196329

Purpose

A yeast-Escherichia coli-Agrobacterium shuttle vector

Depositor

Zhenghe Li

Article

Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

Type

Empty backbone

Expression

Plant

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-G418

Plasmid

#160215

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HPH

Plasmid

#160216

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinA K108A GFP

Plasmid

#32125

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP, HDEL, and Kar2

Expression

Yeast

Mutation

K108A

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-natMX6 (pFB1434)

Plasmid

#126050

Purpose

For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPAP002

Plasmid

#153489

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPAP001

Plasmid

#153488

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRSII42H-TDH3pr

Plasmid

#194533

Purpose

High copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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SIVu

Plasmid

#81089

Purpose

Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-kanMX6

Plasmid

#26882

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1

Plasmid

#41606

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

TRP1 reverse orientation

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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SIVl

Plasmid

#81090

Purpose

Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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SIVt

Plasmid

#81092

Purpose

Single Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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SIVh

Plasmid

#81091

Purpose

Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSH100 (YCplac33 MET25pro MCP-mCherry)

Plasmid

#45930

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

MCP-mCherry

Tags

mCherry

Expression

Yeast

Promoter

Met25

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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EC2_1_dCas9_sgRNA

Plasmid

#163710

Purpose

Yeast low copy plasmid with dCas9 and sgRNA expression cassette

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-kanMX6

Plasmid

#89067

Purpose

genomic GBP epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP

Expression

Yeast

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKT-iRFP-KAN

Plasmid

#64687

Purpose

pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

iRFP

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pXP218

Plasmid

#26831

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pXP220

Plasmid

#26832

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pXP422

Plasmid

#26845

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pXP418

Plasmid

#26843

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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