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We narrowed to 5,049 results for: golden gate

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Showing: 4901 - 4950 of 5049 results

  5. pNK2657

    Plasmid
    #219741
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    hispidin- synthase from Mycena citricolor
    Use
    Synthetic Biology
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219741-plasmid-map-sequence-id-437095

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  17. LentiX-(loxPcon-TET-DIAL-YB-TATA)-mCherry-HRasG12V-bGH-EFS-rtTA-TagBFP-WPRE

    Plasmid
    #246367
    Purpose
    loxPcon TET-DIAL Reporter Lentivirus with YB_TATA expressing mCherry-HRasG12V in the presence of DOX and rtTA and editable by Cre recombinase. Contains rtTA-TagBFP expressed divergenty.
    Depositor
    Kate Galloway
    Article
    Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103
    Insert
    mCherry-GS-HRasG12V (HRAS Human)
    Use
    Lentiviral and Synthetic Biology
    Mutation
    G12V
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    246367-plasmid-map-sequence-id-497484

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  18. pCBS-5391

    Plasmid
    #249330
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and Non-targeting guide for mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249330-plasmid-map-sequence-id-497003

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  19. pCBS-5846

    Plasmid
    #249329
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A)and sg#3 targeting VEGFA gene in mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249329-plasmid-map-sequence-id-497002

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  20. pCBS-6756

    Plasmid
    #249328
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249328-plasmid-map-sequence-id-497001

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  21. pcDNA5-3xFLAG-NRF1-∆ex7_WobbleMut

    Plasmid
    #237456
    Purpose
    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged NRF1 isoform lacking the alternative exon-7 (∆ex7) mutated to be resistant to NRF1 siRNA
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:
    Insert
    NRF1 ∆exon-7
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CMV/TO inducible promoter
    Available Since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    237456-plasmid-map-sequence-id-476325

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  25. L4385 dsRedExpress2 reporter, IL-10Rb NTEVp, IL-10Ra CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244186
    Purpose
    PiggyBac transposon vector for expression of dsRedExpress2 under synTF promoter; constitutive expression of IL-10Rb NTEVp chain, IL-10Ra CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRedExpress2 under synTF responsive promoter; IL-10Rb NTEVp chain with human CD8a SS; IL-10Ra CTEVp chain; mNeonGreen-P2A-PuroR (CD8A Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244186-plasmid-map-sequence-id-489485

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  29. pLCHKOv3-mClover-intergenic_2-As

    Plasmid
    #218814
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218814-plasmid-map-sequence-id-436107

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  30. pLCHKOv3-mClover-intergenic_3-As

    Plasmid
    #218815
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218815-plasmid-map-sequence-id-436108

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  31. pLCHKOv3-mClover-intergenic_4-As

    Plasmid
    #218816
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218816-plasmid-map-sequence-id-436109

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  39. pGGG-AH-VRT-A2

    Plasmid
    #163703
    Purpose
    Wheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)
    Depositor
    Cristobal Uauy
    Article
    Adamski et al Plant Cell. 2021 Aug 13;33(7):2296-2319.
    Inserts
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
    Expression
    Plant
    Promoter
    Rice - Os Actin promoter and Triticum polonicum V…
    Available Since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    163703-plasmid-map-sequence-id-320920

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  42. pNK3071

    Plasmid
    #219755
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Expression
    Plant
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219755-plasmid-map-sequence-id-450717

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  43. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Expression
    Plant
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219754-plasmid-map-sequence-id-458646

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  45. pNK115

    Plasmid
    #219745
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219745-plasmid-map-sequence-id-450697

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  46. pNK116

    Plasmid
    #219742
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Mutation
    D37E, V181I, S323M, M385K
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219742-plasmid-map-sequence-id-437105

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  48. pNK189

    Plasmid
    #219743
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Mutation
    D37E, V181I, S323M, M385K
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219743-plasmid-map-sequence-id-437108

    Enlarge

  49. pNK4169

    Plasmid
    #219744
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Mutation
    D37E, V181I, S323M, M385K
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219744-plasmid-map-sequence-id-437109

    Enlarge

  50. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

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Showing: 4901 - 4950 of 5049 results