We narrowed to 7,564 results for: ALP

1. EGFP-SopB

   Plasmid

   #183657

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL-Blast FAPwt

   Plasmid

   #210643

   Purpose

   Expresses the FAP (Fibroblast Activation Protein) protein.

   Depositor

   Jing Yang

   Article

   Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

   Insert

   Fibroblast Activation Protein Alpha (FAP Human)
   

   Use

   Retroviral

   Mutation

   none

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1-hVAP-A

   Plasmid

   #104447

   Purpose

   Expression of human VAP-A fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO1-102.1F10-IgA1/λ

   Plasmid

   #50370

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW TetO HA-AMPKa1 WT

   Plasmid

   #69824

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TetO-FUW-BICRAL-FLAG-pgk-puro

   Plasmid

   #107732

   Purpose

   Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

   Depositor

   Emily Dykhuizen

   Article

   Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

   Insert

   BICRAL (BICRAL Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PIK3C2A gRNA (BRDN0001149181)

   Plasmid

   #76414

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2A (PIK3C2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PIK3C2A gRNA (BRDN0001148547)

    Plasmid

    #76415

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. FR_HNF4A8

    Plasmid

    #31114

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 8

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid

    #49332

    Purpose

    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

    Promoter

    CMV

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-102.1F10-IgA2/λ

    Plasmid

    #50371

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmcherry α-catenin ΔVBS

    Plasmid

    #178649

    Purpose

    express α-catenin without a vinculin binding site (VBS)

    Depositor

    Daniel Conway

    Article

    Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

    Insert

    α-catenin (CTNNA1 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    VBS of alpha catenin was removed and replaced wit…

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pATZ-BFP-HA-GFP11

    Plasmid

    #177721

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    GFP11, HA, and TagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro

    Plasmid

    #22135

    Depositor

    William Kaelin

    Article

    Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids from wt: Proline 405 t…

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. iSH-iRFP670-sspB

    Plasmid

    #176102

    Purpose

    Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    phosphatidylinositol 3-kinase (Pik3r1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-IpgD(C439S)

    Plasmid

    #183667

    Purpose

    N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C439S

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. α-catenin ECFP-YPET FRET ΔF-actin binding site

    Plasmid

    #198245

    Purpose

    Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
    

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted F-actin binding site on a-catenin (849-90…

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEBG-bZIPa

    Plasmid

    #12436

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    bZIP of C/EBPa (CEBPA Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only