We narrowed to 19,284 results for: Cre

1. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

   Plasmid

   #111388

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

   Plasmid

   #111395

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   DTR-EGFP
   

   Use

   AAV

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

   Plasmid

   #113694

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUCmini-iCAP-PHP.V2

   Plasmid

   #127848

   Purpose

   non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUCmini-iCAP-PHP.B4

   Plasmid

   #127849

   Purpose

   non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.B4 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Syn1-DIO-VGLUT2:miniSOG

   Plasmid

   #214200

   Purpose

   For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

   Depositor

   Thomas Hnasko

   Article

   Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

   Insert

   Slc17a6 tagged with miniSOG and hemagglutinin
   

   Use

   AAV and Cre/Lox

   Tags

   miniSOG (tagged with HA) is fused to the first in…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MultiMate 5 colors

   Plasmid

   #206266

   Purpose

   Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
   

   Use

   Recombinant baculovirus production, cre acceptor…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-DIO-trkB.DN-mCherry

   Plasmid

   #121502

   Purpose

   Expresses trkB.T1 fused with mCherry in a cre dependent manner.

   Depositor

   Marie Carlén

   Article

   Guyon et al J Neurosci. 2021 Feb 15. pii: JNEUROSCI.

   Insert

   trkB.T1 (Ntrk2 Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-IV-GFPL10

   Plasmid

   #98704

   Purpose

   Cre-dependently express GFPL10 fusion using Introvert Switch

   Depositor

   Jeffrey Friedman, Alexander Nectow

   Article

   Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

   Insert

   GFPL10
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L10: 2609 C->T to remove EcoRI site

   Promoter

   EF1a

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-FLEX-ArchT-GFP

    Plasmid

    #58851

    Purpose

    AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105983

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    SYFP2-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin 1

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAVS1 KanR foundation lox casette

    Plasmid

    #132388

    Purpose

    Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    G418 resistance
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    promoterless

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

    Plasmid

    #113681

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

    Plasmid

    #113678

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCALNL-mutKir2.1

    Plasmid

    #125579

    Purpose

    Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A non-conducting mutant (GYG -> AAA).

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

    Plasmid

    #111387

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid

    #105982

    Purpose

    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    

    Use

    AAV and Cre/Lox

    Tags

    FAPdL5-POST, T2A-dTomato, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin 1

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge