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pMT2-HA-RAPGEF2(S1244A/S1248A)

Plasmid

#110163

Purpose

Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S1244A/S1248A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (WT)

Plasmid

#42202

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

c-SRC (SRC Human)

Use

Tags

Expression

Mammalian

Mutation

L176F mutation compared to reference sequence NM_…

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_NESPylRS(AF)_hU6tRNAPyl

Plasmid

#223508

Purpose

PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

Inserts

NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
tRNA Pyl

Use

Tags

Expression

Mammalian

Mutation

Y306A; Y384F

Promoter

CVM, SP6 and U6

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-membrane-mEGFP

Plasmid

#105526

Purpose

T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

GNAI2 (GNAI2 Human)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

fragment encoding the plasma membrane targeting s…

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_NESPylRS(WT)_hU6tRNAPyl

Plasmid

#223509

Purpose

PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

Inserts

NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
tRNA Pyl

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, SP6 and U6

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#101061

Purpose

AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2-F391W

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2-P2A-BSD

Plasmid

#174818

Purpose

Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2-P2A-BSD

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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OPEN Beta2-microglobulin

Plasmid

#215657

Purpose

E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN Beta2-microglobulin (B2M Human)

Use

Tags

Expression

Bacterial

Mutation

Histidine 31 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mutation

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-MRLC1-mRuby2-IRES-PuroR

Plasmid

#103031

Purpose

Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

mRuby2

Expression

Mutation

Promoter

CMV immediate early

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon optimized C-terminal 230 amino acids

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short W284A

Plasmid

#72551

Purpose

expresses 3*FLAG tagged human NSD3-short with W284A mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

W284A

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(1-100)

Plasmid

#72547

Purpose

expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

1-100 aa deletion

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A

Plasmid

#238333

Purpose

proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Use

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-R372A-H373A-R376A

Promoter

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A

Plasmid

#238338

Purpose

proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Use

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-P378V-G500V-G501A

Promoter

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A

Plasmid

#238339

Purpose

proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Use

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-R722S-D724A-T726A

Promoter

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A

Plasmid

#238340

Purpose

proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Use

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-K383A-K447A-K598A-R722A

Promoter

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A

Plasmid

#238334

Purpose

proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Use

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-R372A-H373A-R376A-E882A

Promoter

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A

Plasmid

#238341

Purpose

proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Use

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-K379A-K381A-K498A

Promoter

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge