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We narrowed to 6,501 results for: nls

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Showing: 1961 - 1980 of 6501 results

  2. nDHFR173-gs10-NLS-VPR

    Plasmid
    #188262
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nDHFR173 (DHFR Human)
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188262-plasmid-map-sequence-id-374570

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  3. nGR2175-gs10-NLS-VPR

    Plasmid
    #188264
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Publication
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nGR2175
    Expression
    Mammalian
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188264-plasmid-map-sequence-id-374534

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  4. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    84239-plasmid-map-sequence-id-160811

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  8. pDN77

    Plasmid
    #61347
    Purpose
    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    GS linker-AsLov2-biNLS2 and NES IkBa
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    61347-plasmid-map-sequence-id-102095

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  10. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  15. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107318
    Purpose
    Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107318-plasmid-map-sequence-id-204674

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  16. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid
    #107320
    Purpose
    Expresses SpCas9 fused to SaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to SaCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    107320-plasmid-map-sequence-id-204686

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  18. pCAG-All-in-one-hCascade

    Plasmid
    #134919
    Purpose
    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    134919-plasmid-map-sequence-id-272938

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  19. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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Showing: 1961 - 1980 of 6501 results