We narrowed to 2,414 results for: organization Addgene

1. H3K9me3 biosensor

   Plasmid

   #120802

   Purpose

   FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-TRE3G-FNLS-PGK-Puro

   Plasmid

   #110847

   Purpose

   Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   TRE3G

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PMP34-mApple

   Plasmid

   #214417

   Purpose

   Peroxisome localization of PMP34 fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. H3S10ph biosensor

   Plasmid

   #120809

   Purpose

   FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Threonine 3, Threonine 6, and Threonine 11 are mu…

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/tom20-pbira(79-c)-ha

   Plasmid

   #154456

   Purpose

   expresses tom20-pbira(79-c)-ha/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   tom20-pbira(79-c)-ha (TOMM20 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TPC2(N257A)-mCherry

   Plasmid

   #135186

   Purpose

   Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Mutation of asparagine257 to alanine

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/mCherry_pBirA-HA_FKBP8

   Plasmid

   #154457

   Purpose

   expresses mCherry_pBirA-HA_FKBP8/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   mCherry_pBirA-HA_FKBP8 (FKBP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TPC1(ΔN69)-mCherry

    Plasmid

    #135184

    Purpose

    TPC1 with truncated N-terminus, tagged with mCherry on C terminus.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC1 (Tpcn1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-69

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TPC2(ΔN49)-mCherry

    Plasmid

    #135185

    Purpose

    TPC2 with truncated N-terminus, tagged with mCherry on C terminus.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-49

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-CMV-mouse Gfi1-GFP

    Plasmid

    #91892

    Purpose

    3rd generation lentiviral expression of mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3/FKBP12-pBirA(284-C)-HA

    Plasmid

    #154452

    Purpose

    expresses FKBP12-pBirA(284-C)-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    FKBP12-pBirA(284-C)-HA (FKBP12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3/FKBP12-pBirA(79-C)-ha

    Plasmid

    #154449

    Purpose

    expresses FKBP12-pBirA(79-C)-ha/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    FKBP12-pBirA(79-C)-ha (FKBP12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge