We narrowed to 2,314 results for: pcas9

1. ABE8e(TadA-8e K20A R21A)

   Plasmid

   #138496

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e K20A R21A)-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA-8e K20A R21A

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPQD1CB0202

   Plasmid

   #185626

   Purpose

   MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-sgRNA_Dicer_3

   Plasmid

   #68809

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px458-Cas9n-Trp63-Exon4-2sgRNA

   Plasmid

   #88849

   Purpose

   CRISPR KO of Trp63

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp63 (Trp63 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

   Plasmid

   #159658

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

   Plasmid

   #159657

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT45_GalL_nCDA1-BE3

   Plasmid

   #145038

   Purpose

   Expresses nCDA1-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAEL007584-Cas9

    Plasmid

    #100706

    Purpose

    The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007584-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL007584 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pJT79_GalL_nCDA1Δ192-BE3

    Plasmid

    #145045

    Purpose

    Expresses nCDA1Δ192-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ192-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-192AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Px458-Cas9n-Trp73-Exon4-3sgRNA

    Plasmid

    #88850

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Px458-Cas9n-Trp73-Exon4-4sgRNA

    Plasmid

    #88851

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pLKO1-puro-U6-sgRNA-LRTM2

    Plasmid

    #69237

    Purpose

    U6 driven SpCas9 sgRNA expression for LRTM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    LRTM2 sgRNA (LRTM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. EHMT1_exon 3_gRNA

    Plasmid

    #228809

    Purpose

    CRISPR targeting of human EHMT1

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    gRNA targeting EHMT1 exon 3 (EHMT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pECNUS4_MP

    Plasmid

    #228358

    Purpose

    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-tevopreq1-FXR1-G266E

    Plasmid

    #225482

    Purpose

    Express epegRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    epegRNA for editing FXR1-G266E (FXR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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