165,744 results

1. TARS_pNIC-Bio3

   Plasmid

   #153057

   Purpose

   Expression construct for producing Avi tagged Threonyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   TARS (TARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad-PhoCl2f

   Plasmid

   #164036

   Purpose

   Improved PhoCl variant with faster dissociation rate

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2f
   

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Promoter

   araBad promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-LSS-SGFP2

   Plasmid

   #112943

   Purpose

   Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Insert

   LSS-GFP2 mCherry fusion
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a MDHP_YEAST

   Plasmid

   #204233

   Purpose

   Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHP_YEAST (MDH3 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   The last 3 C-terminal residues (SKL) are the pero…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. NanoLuc-PM-Venus

   Plasmid

   #164786

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
   

   Tags

   NanoLuc and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-sgRNA expression vector

   Plasmid

   #51132

   Purpose

   For in vitro transcription of sgRNA from the T7 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

   Plasmid

   #84471

   Purpose

   pAAV-RAM-ChR2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   Channelrhodopsin 2
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R

   Promoter

   TRE

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lifeact-3xmNG::HIS3

   Plasmid

   #227596

   Purpose

   Integration vector for expressing Lifeact-3xmNeonGreen

   Depositor

   Bruce Goode

   Article

   Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

   Insert

   Lifeact-3xmNeonGreen::HIS3
   

   Tags

   3xmNeonGreen

   Expression

   Yeast

   Mutation

   Truncated ABP140 (aa 1-17 only)

   Promoter

   Abp140

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_TNFRSF17_WT

   Plasmid

   #82263

   Purpose

   Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TNFRSF17 (TNFRSF17 Human)
   

   Use

   Gateway entry vector

   Mutation

   N81S; K86E

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPP381

    Plasmid

    #158798

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-2
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RuvC-inactivating mutation D394A

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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