8,139 results

1. pGKB

   Plasmid

   #85077

   Purpose

   expression in Pichia pastoris

   Depositor

   Sheng Yang

   Article

   Yang et al Yeast. 2014 Apr;31(4):115-25.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCYC1m_yeGFP

   Plasmid

   #64389

   Purpose

   encodes yeast enhanced GFP under control of minimal pCYC1 promoter

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced GFP
   

   Expression

   Yeast

   Promoter

   pCYC1

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKanB

   Plasmid

   #223523

   Purpose

   Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

   Depositor

   Geoff Lin-Cereghino

   Article

   Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Sept. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pND003

   Plasmid

   #200949

   Purpose

   Yeast surface display destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Yeast surface display

   Tags

   AGA2, HA, and Myc

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC-humanADAR1

   Plasmid

   #198235

   Purpose

   Expresses human ADAR1 in yeast

   Depositor

   Brenda Bass

   Article

   Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

   Insert

   human ADAR1 (ADAR Human)
   

   Tags

   His10_TEV

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Tags

   ytdTomato

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVT36b

   Plasmid

   #184437

   Purpose

   CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

   Depositor

   Huimin Zhao

   Article

   Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. YEp181-CUP1-His-Ubi

   Plasmid

   #99539

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Tags

   7xHis

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRP2371

   Plasmid

   #127577

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Yeast

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRP2374

    Plasmid

    #127579

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Yeast

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM0641

    Plasmid

    #129006

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. YIplac211-iGFP-PIK1

    Plasmid

    #105260

    Purpose

    Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Pik1 (PIK1 Budding Yeast)
    

    Tags

    iGFP

    Expression

    Yeast

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2797

    Plasmid

    #63639

    Purpose

    pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB2875

    Plasmid

    #63640

    Purpose

    pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB2989

    Plasmid

    #63636

    Purpose

    pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 413-Met25 Kar2 TorsinA E171Q GFP

    Plasmid

    #32127

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    E171Q

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

17. 413-Met25 Kar2 TorsinA K108A GFP

    Plasmid

    #32125

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Tags

    GFP, HDEL, and Kar2

    Expression

    Yeast

    Mutation

    K108A

    Available Since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP082

    Plasmid

    #103875

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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