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pZS1[GalS-L,FruR-L]

Plasmid

#60764

Purpose

Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
FruR-L

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Promoter

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,TreR-L]

Plasmid

#60765

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
TreR-L

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Promoter

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L,TreR-L]

Plasmid

#60766

Purpose

Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-L

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1[RbsR-T,LacI-T]

Plasmid

#60767

Purpose

Contains PIq driving expression RbsR-T, and PI driving expression of TreR-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
Dimeric LacI with the TAN DBD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Dimeric LacI with the TAN DBD. and LacI/GalR repr…

Promoter

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-T,LacI-T]

Plasmid

#60768

Purpose

Contains PIq driving expression FruR-T, and PI driving expression of LacI-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-T
Dimeric LacI with the TAN DBD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Dimeric LacI with the TAN DBD. and LacI/GalR repr…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1[TreR-T,LacI-T]

Plasmid

#60770

Purpose

Contains PI driving expression TreR-T, and PI driving expression of LacI-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

TreR-T
Dimeric LacI with the TAN DBD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Dimeric LacI with the TAN DBD. and LacI/GalR repr…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1[FruR-T,TreR-T]

Plasmid

#60772

Purpose

Contains PIq driving expression FruR-T, and PIq driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-T
TreR-T

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with FruR LB…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1[GalS-T,RbsR-T]

Plasmid

#60773

Purpose

Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-T
GalS-T

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with GalS LB…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1[GalS-T,TreR-T]

Plasmid

#60775

Purpose

Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-T
TreR-T

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with GalS LB…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1[RbsR-T,TreR-T]

Plasmid

#60776

Purpose

Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

RbsR-L
TreR-T

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. TAN DBD with RbsR LB…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-25BoxB

Plasmid

#60817

Purpose

Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

Depositor

Maria Carmo-Fonseca

Article

Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

Insert

25x BoxB

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

25 repeats of BoxB sequence

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#60847

Purpose

Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

Depositor

Jamie Cate

Article

Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

Inserts

S. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA

Use

CRISPR and Synthetic Biology

Tags

NLS/His8 and TTT 3' extension prior to sgRNA

Expression

Bacterial and Yeast

Mutation

L4 is UUCG tetraloop and guide targets LYP1 (CATA…

Promoter

Availability

Academic Institutions and Nonprofits only

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pTH

Plasmid

#56659

Purpose

Dual-expression vector for P. tricornutum

Depositor

Philip Weyman

Article

Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

Insert

KanR

Use

Synthetic Biology; Diatom expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Gal4pBS12-Pleum

Plasmid

#60326

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4pBS24-Pleum

Plasmid

#60327

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
UASGAL-CU2-Pleum

Plasmid

#60328

Purpose

Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4pBS3-Pleum

Plasmid

#60330

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
UASGAL-CU1-Pleum

Plasmid

#60331

Purpose

Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge